"

From 2010.igem.org

Revision as of 21:11, 27 October 2010 (view source) Onoda (Talk | contribs) (→Results) ← Older edit		Revision as of 21:16, 27 October 2010 (view source) Onoda (Talk | contribs) (→Results) Newer edit →
Line 171:		Line 171:
	==Results==		==Results==
	AHLのありおよびなしに関わらず蛍光が観察された。<br>		AHLのありおよびなしに関わらず蛍光が観察された。<br>
		+	We confirmed<br>
	[[Image:Plux inv results.png|frame|right|Fig. 3 ]]		[[Image:Plux inv results.png|frame|right|Fig. 3 ]]

---

Revision as of 21:16, 27 October 2010

Contents 1 Abstract 2 Experiments 2.1 1.Preparing Plux inv-GFP 2.2 2.Characterization Plux inv-GFP 3 Results 4 Reference

Abstract

LuxR is an AHL-dependent activator. However to insert lux box between -35 and -10 and LuxR fuctions as LuxR-AHL inverter. Someone resistered the Plux inverter in Biobrick.We've prepare Plux inv-GFP and characterized about it.

Experiments

1.Preparing Plux inv-GFP

1．Plux inv－GFPの作製 クローニングのprocessをFig. 1に示す。作製したPlux inv-GFPを形質転換した株は緑色蛍光を示した。また，sequenceも正しいことを確認した。 Preparing Plux inv-GFP process is shown in Fig. 1.

2.Characterization Plux inv-GFP

Plux inv-GFP およびLuxR generatorである Plac-LuxRをDH10B株に共形質転換した。コロニーをつついてLB液体培地(AHL0および1000 nM)で12 h培養し, スピンダウンしてペレットを観察した。 We cotransformded Plux inv-GFP and LuxR generator,Plac-LuxR in DH10B.

Results

AHLのありおよびなしに関わらず蛍光が観察された。
We confirmed

Reference

1. Egland.K.A, and Greenberg.E.P, Conversion of the Vibrio Fischeri Transcriptional Activator,LuxR, to a Repressor, J. Bacteriol., 182, P.805-811 (2000)
2. Cox.R.S.3rd, Surette.M.G, Elowitz.M.B, Programming gene expression with combinatorial promoters, Mol Syst Biol, 3 ,145 (2007)