Team:Heidelberg/Notebook/BSDesign/October

From 2010.igem.org

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(→10/02/2010)
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*1µg digestion of miMeasure and 6 different constructs (1A, 3F, 13, 15, 17, 37) with EcoRI and PstI
*1µg digestion of miMeasure and 6 different constructs (1A, 3F, 13, 15, 17, 37) with EcoRI and PstI
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1= 0S
+
:1= 0S
-
2= 0L
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:2= 0L
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3= 10S
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:3= 10S
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4= 10L
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:4= 10L
20 Miniprep:
20 Miniprep:
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|20||1179||4.8
|20||1179||4.8
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{{:Team:Heidelberg/Single_Bottom}}
{{:Team:Heidelberg/Single_Bottom}}

Revision as of 20:48, 27 October 2010
 


Binding Site Design - October

10/02/2010


  • digestion in 50 µl of
    • 4x pSB1C3 (5µl BSA, 5µl NEB buffer 2, 1µl PstI, 1µl EcoRI, 1µl DpnI, 4µl construct, 33 µl H2O)
    • 4x pSB1A3 (5µl BSA, 5µl NEB buffer 2, 1µl PstI, 1µl EcoRI, 1µl DpnI, 4µl construct, 33 µl H2O)

10/05/2010


  • 1µg digestion of miMeasure and 6 different constructs (1A, 3F, 13, 15, 17, 37) with EcoRI and PstI
1= 0S
2= 0L
3= 10S
4= 10L

20 Miniprep:

Table 1: 20 Minis
Numberbinding site against miRNA xPosition
12213.5
22211.4
311793.1
442862.6
542864.1
642863.4
711794.4
83.5
911791.9
102214.6
112211.5
1242863.5
132214.8
142212.3
1542862.2
1642861.3
1711792.5
1811792.4
1911791.5
2011794.8

Retrieved from "http://2010.igem.org/Team:Heidelberg/Notebook/BSDesign/October"