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Team:Newcastle/23 June 2010
From 2010.igem.org
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=== Wednesday afternoon taking a break === | === Wednesday afternoon taking a break === | ||
| + | |||
| + | ====Aims==== | ||
| + | The aim of this Lab practice was to have fun. | ||
| + | |||
| + | ====Equipement list==== | ||
| + | * Candy floss | ||
| + | * Suntan lotion | ||
| + | * Teddy bears | ||
| + | * Burgers | ||
| + | * Chips and gravy | ||
| + | * Meerkat toys | ||
| + | * Madmouse ride! | ||
| + | * Beer, wine, lemonade | ||
| + | * Football | ||
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Revision as of 10:49, 12 July 2010
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Contents |
Wednesday Morning
Aims
The aim of today's Lab practice was to transform competent cells with GFP and RFP inserts in an RFP backbone.
Equipement List
- E.coli broth culture
- Pipettes
- Plasmid ligation products from yesterday
- Conical flasks
Transformation
Cells are made competent ready for transformation.
- Positive control(plasmid uncut)- to make sure cells are transfroming properly
- Negative control (just cells)- nothing but competent cells
5 microfuge tubes:
- 1:5
- 1:3
- Vector
- Negative add nothing
- Positive add 1μl uncut
Wednesday afternoon taking a break
Aims
The aim of this Lab practice was to have fun.
Equipement list
- Candy floss
- Suntan lotion
- Teddy bears
- Burgers
- Chips and gravy
- Meerkat toys
- Madmouse ride!
- Beer, wine, lemonade
- Football
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