Team:Osaka/week12

From 2010.igem.org

(Difference between revisions)
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#Transformation
#Transformation
#*048,060,051,066,069,070,1-13D
#*048,060,051,066,069,070,1-13D
 +
 +
==October 11 (Mon)==
 +
 +
#Transformation
 +
#*034,031,027,029,075
 +
#*014-2,021-2,022-2,025-2,047
 +
#mini prep
 +
#*1-23L,1-12M,061①,064-2②,056①②,013①②
 +
#*1-13D,061②,064-2①  →　destruction
 +
#cut check
 +
#*1-12M,064-2②,013①,056①,061①,1-23L
 +
#To liquid culture
 +
#*plate 025(10/8),022(10/8) each 2 colony to culture
 +
#Electrophoresis 
 +
#*064  ok
 +
#*061    x
 +
#*056    x
 +
#*013    ok
 +
#Ligation
 +
#*048(C) R1 + FcenA
 +
#*051(C) R2 + FcenA
 +
#*053(C) R2 + 024
 +
#*059(C) 001-2 + ToR
 +
#*068(K) 050 + 026
 +
#*070(A) 052 + 054
 +
#*072(K) 049 + 054
 +
#*076(A) 050 + 054
 +
#*077(A) 2-4A + R1
 +
#cutcheck
 +
#*R2 →XP
 +
#*1-23L→ E
 +
#To liquid culture
 +
#*1-13D①,070①②,066①②,069①②,060①②,048①②,051①②
 +
#Electrophoresis
 +
#*1-23L  ok
 +
#*036②  ok
 +
#Ligation
 +
#*086(C) 024 + 1-23L
 +
#*078(A) 2-4A + R2
 +
#*085(C) 023 + 1-23L
 +
#*084(K) 049 + 1-23L
 +
#*083(K) 3-2P + 1-23L
 +
#*082(K) 035 + 1-23L
 +
#*081(C) 022 + 1-23L
 +
#*079(C) 036 + 1-23L
 +
 +
==October 12 (Tue)==
 +
#mini prep
 +
#*1-13D,022-2①②,048①②,060①②,066①②,069①②,070①②
 +
#*051①→　destrustion
 +
#Transformation
 +
#*047,063,069,0071,078,079,080,081,082,083,084,085
 +
#cutcheck
 +
#*1-13D,022-2,048,066,069,070,1-13D,060
 +
#Electrophoresis
 +
#*1-13D  →x
 +
#*1-13  →x
 +
#*022-2  →x
 +
#*048    →OK
 +
#*060    →?
 +
#*066    →OK
 +
#*069    →OK
 +
#*070    →OK
 +
#To liquid culture
 +
#*034,029,027,019-2,022-2,025-2,021-2,031,075,051,072,070,068,053,059,077,076,048,060,1-23L
 +
#cutcheck
 +
#*1-23L,048
 +
#Electrophoresis
 +
#.Ligation
 +
#*030(C) SUC d + 1-3A
 +
#*087(A) 048 + 026
 +
#*088(A) 048 + 054
 +
#*089(C) 2-4A + 054
 +
 +
==October 13 (Wed)==

Revision as of 20:31, 27 October 2010


October 10 (Sun)

  1. Electrophoresis
  2. Ligation
    • 048(C) R1 + FcenA
    • 060(C) 001-2 + GeneⅢ
    • 051(C) R2 + 022
    • 066(A) 3-2P + 026
    • 069(A) 049 + 026
    • 070(A) 052 + 026
  3. mini prep
    • 058①,051②,016①,013②
  4. cut check
    • 016①,058①,013②,051②,1-13D,050①,025②
    • PCR Products(ADH2,ENO1)
    • Man→034
    • glr→031
    • ADH2→027
    • ENO→029
    • Cel44A→075
  1. PCR for DNA segment
    • Cel5B 3/4 α-agultinin
  2. To liquid culture
    • 1-13D,1-12M,1-23L,013,056,069-2,061 →culture
  3. Transformation
    • 048,060,051,066,069,070,1-13D

October 11 (Mon)

  1. Transformation
    • 034,031,027,029,075
    • 014-2,021-2,022-2,025-2,047
  2. mini prep
    • 1-23L,1-12M,061①,064-2②,056①②,013①②
    • 1-13D,061②,064-2① →　destruction
  3. cut check
    • 1-12M,064-2②,013①,056①,061①,1-23L
  4. To liquid culture
    • plate 025(10/8),022(10/8) each 2 colony to culture
  5. Electrophoresis
    • 064 ok
    • 061 x
    • 056 x
    • 013 ok
  6. Ligation
    • 048(C) R1 + FcenA
    • 051(C) R2 + FcenA
    • 053(C) R2 + 024
    • 059(C) 001-2 + ToR
    • 068(K) 050 + 026
    • 070(A) 052 + 054
    • 072(K) 049 + 054
    • 076(A) 050 + 054
    • 077(A) 2-4A + R1
  7. cutcheck
    • R2 →XP
    • 1-23L→ E
  8. To liquid culture
    • 1-13D①,070①②,066①②,069①②,060①②,048①②,051①②
  9. Electrophoresis
    • 1-23L ok
    • 036② ok
  10. Ligation
    • 086(C) 024 + 1-23L
    • 078(A) 2-4A + R2
    • 085(C) 023 + 1-23L
    • 084(K) 049 + 1-23L
    • 083(K) 3-2P + 1-23L
    • 082(K) 035 + 1-23L
    • 081(C) 022 + 1-23L
    • 079(C) 036 + 1-23L

October 12 (Tue)

  1. mini prep
    • 1-13D,022-2①②,048①②,060①②,066①②,069①②,070①②
    • 051①→　destrustion
  2. Transformation
    • 047,063,069,0071,078,079,080,081,082,083,084,085
  3. cutcheck
    • 1-13D,022-2,048,066,069,070,1-13D,060
  4. Electrophoresis
    • 1-13D →x
    • 1-13 →x
    • 022-2 →x
    • 048 →OK
    • 060 →?
    • 066 →OK
    • 069 →OK
    • 070 →OK
  5. To liquid culture
    • 034,029,027,019-2,022-2,025-2,021-2,031,075,051,072,070,068,053,059,077,076,048,060,1-23L
  6. cutcheck
    • 1-23L,048
  7. Electrophoresis
  8. .Ligation
    • 030(C) SUC d + 1-3A
    • 087(A) 048 + 026
    • 088(A) 048 + 054
    • 089(C) 2-4A + 054

October 13 (Wed)

Retrieved from "http://2010.igem.org/Team:Osaka/week12"