Team:USTC/Project/protein/conclusion

From 2010.igem.org

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==''' Part Ⅰ: Fusion Proteins to Identify the Localization of BMC '''==
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From the following picture(Fig 1), we can identify the structure and Localization of BMC.
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[[Image:USTC2010_Results_of_Fusion_Protein-RFP-A(1).jpg]]
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  Fig 1: Results of fusion protein --- RFP-A
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==''' Part Ⅱ: Fusion Proteins for Transportation into BMC''' ==
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The series of studies presented here demonstrates that a short N-terminal peptide is necessary and sufficient for association of proteins with BMC and likely mediates packaging within the interior of BMC.
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[[Image:USTC2010_Result_of_Fusion_Protein_P_L-GFP(1).jpg]]
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[[Image:USTC2010_Result_of_Fusion_Protein_P_L-GFP(2).jpg]]
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[[Image:USTC2010_Result_of_Fusion_Protein_P_L-GFP(3).jpg]]
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[[Image:USTC2010_Result_of_Fusion_Protein_P_L-GFP(4).jpg]]
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  Fig 2: Results of fusion protein --- PduP_L-GFP
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== '''Part Ⅲ: Fusion Proteins for BMC Purification''' ==
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The localization of BMC can be observed in Fig 3, which is expressed by the fusion protein of PduV[1-98] and GFP.
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[[Image:USTC2010_Results_of_Fusion_Protein_V_S-GFP(1).jpg]]
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  Fig 3: Results of fusion protein --- PduV_S-GFP

Latest revision as of 18:24, 27 October 2010

An Integrated Platform Based on Bacterial Microcompartment for de novo Proteinaceous Artificial Organelles
















Part Ⅰ: Fusion Proteins to Identify the Localization of BMC

From the following picture(Fig 1), we can identify the structure and Localization of BMC.

USTC2010 Results of Fusion Protein-RFP-A(1).jpg 

 Fig 1: Results of fusion protein --- RFP-A

Part Ⅱ: Fusion Proteins for Transportation into BMC

The series of studies presented here demonstrates that a short N-terminal peptide is necessary and sufficient for association of proteins with BMC and likely mediates packaging within the interior of BMC.

USTC2010 Result of Fusion Protein P L-GFP(1).jpg USTC2010 Result of Fusion Protein P L-GFP(2).jpg USTC2010 Result of Fusion Protein P L-GFP(3).jpg USTC2010 Result of Fusion Protein P L-GFP(4).jpg 

 Fig 2: Results of fusion protein --- PduP_L-GFP

Part Ⅲ: Fusion Proteins for BMC Purification

The localization of BMC can be observed in Fig 3, which is expressed by the fusion protein of PduV[1-98] and GFP.

USTC2010 Results of Fusion Protein V S-GFP(1).jpg

Fig 3: Results of fusion protein --- PduV_S-GFP
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