Team:Heidelberg/Notebook/MiMeasure/October

From 2010.igem.org

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Revision as of 17:12, 27 October 2010

July
MTWTFSS
1234
567891011
12131415161718
19202122232425
262728293031

-


August
MTWTFSS
1
2345678
9101112131415
16171819202122
23242526272829
3031


September
MTWTFSS
12345
6789101112
13141516171819
20212223242526
2728293031

-


October
MTWTFSS
123
45 678910
11131415161718
19202122232425
2627282930

-


 


21/10/2010

seeding HeLa and Huh7 in 96-well plates

22/10/2010

Transfection

23/10/2010

seeding HeLa and Huh7 in 96-well plates

24/10/2010

HeLa cells were transfected with miMeasure constructs with GFP expression regulated by different imperfect binding sites for miRsAg. As controls we used miMeasure construct with perfect binding site and no binding site. Each miMeasure was cotransfected with pcDNA plasmid expressing synthetic miRsAg and pcDNA5 expressing synthetic shRNA3.


Transfection of HeLa and Huh7 cells with miMeasure with binding sites for miR122.

Huh7 were transfected with 50ng of miMeasure. HeLa cells were used as a control they were transfected with miMeasure constructs and miR122. Control without miR122 expression plasmid was made.

26/10/2010

microscopy measurements

different binding sites for miRsAg
different binding sites for miR122, HeLa cotransfected with miR122 expression plasmid
different binding sites for miR122, Huh7 cells

Flow cytometry measurements

GFP/BFP ratio of single cells according to flow cytometry analysis

Retrieved from "http://2010.igem.org/Team:Heidelberg/Notebook/MiMeasure/October"