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Team:Tsinghua/Notebook/14 October 2010
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Lonelyroads (Talk | contribs) (New page: ==Group 1A== Select a few clones with tet in genome and incuble to 0.6 OD600. Make competent of this strain for electrotransformation.) |
Lonelyroads (Talk | contribs) (→Group 1A) |
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==Group 1A== | ==Group 1A== | ||
Select a few clones with tet in genome and incuble to 0.6 OD600. Make competent of this strain for electrotransformation. | Select a few clones with tet in genome and incuble to 0.6 OD600. Make competent of this strain for electrotransformation. | ||
| + | Transform helper plasmid (HP) to the competent. Spread to spectinomycin plates and incubate at 30℃. | ||
Latest revision as of 15:46, 27 October 2010
Group 1A
Select a few clones with tet in genome and incuble to 0.6 OD600. Make competent of this strain for electrotransformation. Transform helper plasmid (HP) to the competent. Spread to spectinomycin plates and incubate at 30℃.
