Team:Lethbridge/Project/Catechol Degradation
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- | We will then use our <html><a href="https://2010.igem.org/Team:Lethbridge/Project/Compartamentalization"><font color="#00DC00">lumazine synthase (LS) microcompartment</font></a></html> to isolate the xylE protein. The LS has been previously characterized and shown that it is able to encapsulate other molecules. We can target a protein into the LS through selectively mutating five of the interior amino acids of the LS to glutamate. By attaching a positively charged arginine tag to the C-terminus of the protein for targeting you can selectively target the tagged protein into the compartment (<html><a href="https://2009.igem.org/Team:Lethbridge/Modeling" target="new"><font color="#00DC00">Lethbridge 2009 Modeling</font></a></html>) (Seebeck et al., 2006). We will then purify the complex and use for application in tailings ponds water. Once we have demonstrated that we are successfully able to isolate the xylE we will then add more enzymes for the bioremediation of the tailings ponds. | + | We will then use our <html><a href="https://2010.igem.org/Team:Lethbridge/Project/Compartamentalization"><font color="#00DC00">lumazine synthase (LS) microcompartment</font></a></html> to isolate the xylE protein. The LS has been previously characterized and shown that it is able to encapsulate other molecules. We can target a protein into the LS through selectively mutating five of the interior amino acids of the LS to glutamate. By attaching a positively charged arginine tag to the C-terminus of the protein for targeting you can selectively target the tagged protein into the compartment (<html><a href="https://2009.igem.org/Team:Lethbridge/Modeling" target="new"><font color="#00DC00">Lethbridge 2009 Modeling</font></a></html>) (Seebeck <i>et al.</i>, 2006). We will then purify the complex and use for application in tailings ponds water. Once we have demonstrated that we are successfully able to isolate the xylE we will then add more enzymes for the bioremediation of the tailings ponds. |
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