Team:Chiba/lux promoter

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Revision as of 14:29, 27 October 2010

Abstract

We've charaterized Plux inverter.
There is promoter (HSL-luxR repressor), biobrick number R0061.
We've prepare Plux inv-GFP and characterized about it.

Experiments

1.Preparing Plux inv-GFP
Preparing Plux inv-GFP process is shown in '''Fig. 1'''.GFPの蛍光が確認されたので，Plux inv-GFPは機能している。 and sequence is conformed.

Fig. 1 cloning process

2.Characterization Plux inv-GFP

Fig. 2 assay process

Plux inv-GFP およびLuxR generatorである Plac-LuxRをDH10B株に共形質転換した。

Results

Reference

1) Egland.K.A, and Greenberg.E.P, Conversion of the ''Vibrio Fischeri'' Transcriptional Activator,LuxR, to a Repressor, ''J. Bacteriol.'', '''182''', P.805-811 (2000)
2) Cox.R.S.3rd, Surette.M.G, Elowitz.M.B, Programming gene expression with combinatorial promoters, ''Mol Syst Biol'', '''3''' ,145 (2007)

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