Team:Heidelberg/Notebook/Material/Primer

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Revision as of 13:53, 27 October 2010

Primer Table

ID	Name	Sequence (5' to 3')	amplified	origin	for backbone
001	Casp8FWEcoR1	ttttgaattcatggacttcagcagaaatc	Caspase8 death gene	Caspase8
002	CMV_AgeI_fw	ttttaccggtagaatctgcttagggttagg	CMV, shRNA	pcDNA5/FRT-shRNA	pTR-UF3
003	CMVshRNApA_BglII_rv	taatagatctcagaagccatagagcccacc	CMV, shRNA	pcDNA5/FRT-shRNA	pTR-UF3
004	EGFPRVFse1	ttttggccggcccttgtacagctcgtccatg	EGFP	GFP
005	ElenasFirstSyntheticRNA_F	aatatgtctaaactattatttatgccaaccagccaatctagctactgctaggc	part 2 Elenas first synthetic shRNA	miRsAg	pC-DNA5
006	ElenasFirstSyntheticRNA_R	aataatagtttagacatatttatgccagccagccagaccagctctgctaagg	part 3 Elenas first synthetic shRNA	miRsAg	pC-DNA5
007	ElenasSecondSyntheticRNA_F	gacatgtctaaactattgtcttcggtagcgtcgtagactagctactgctaggc	part 2 Elenas second synthetic shRNA	miRsAg	pC-DNA5
008	ElenasSecondSyntheticRNA_R	gacaatagtttagacatgtcttcggtatcgtcgtatcccagctctgctaagg	part 3 Elenas second synthetic shRNA	miRsAg	pC-DNA5
009	ElenasThirdSyntheticRNA_F	tatttgtctaaactataataattcgcggctggcctgactagctactgctaggc	part 2 Elenas third synthetic shRNA	miRsAg	pC-DNA5
010	ElenasThirdSyntheticRNA_R	tattatagtttagacaaataattcgcgtctggccttcccagctctgctaagg	part 3 Elenas third synthetic shRNA	miRsAg	pC-DNA5
011	ElenasFifthSyntheticRNA_F	tatttgtctaaactataataattcgcggctggcctgactagctactgctaggc	part 2 Elenas fifth synthetic shRNA	miRsAg	pC-DNA5
012	ElenasFifthSyntheticRNA_R	atctatagtttagacaagatgaaacgccgagttaacgccagctctgctaagg	part 3 Elenas fifth synthetic shRNA	miRsAg	pC-DNA5
013	ElenasSixthSyntheticRNA_F	ttattgtctaaactatataactgccgtaactccaaatctagctactgctaggc	part 2 Elenas sixth synthetic shRNA	miRsAg	pC-DNA5
014	ElenasSixthSyntheticRNA_R	ttatatagtttagacaataactgccgtcactccaacgccagctctgctaagg	part 3 Elenas sixth synthetic shRNA	miRsAg	pC-DNA5
015	ElenasSeventhSyntheticRNA_F	atcttgtctaaactatagataactgccatcactccccctagctactgctaggc	part 2 Elenas seventh synthetic shRNA	miRsAg	pC-DNA5
016	ElenasSeventhSyntheticRNA_R	atctatagtttagacaagataactgccgtcactccaaccagctctgctaagg	part 3 Elenas seventh synthetic shRNA	miRsAg	pC-DNA5
017	ElenasEighthSyntheticRNA_F	tacatgtctaaactattgtagtcggttcatgcagcccctagctactgctaggc	part 2 Elenas eighth synthetic shRNA	miRsAg	pC-DNA5
018	ElenasEighthSyntheticRNA_R	tacaatagtttagacatgtagtcggtttatgcagcaaccagctctgctaagg	part 3 Elenas eighth synthetic shRNA	miRsAg	pC-DNA5
019	ElenasNinethSyntheticRNA_F	aaattgtctaaactatatttgatccagagatacagaactagctactgctaggc	part 2 Elenas nineth synthetic shRNA	miRsAg	pC-DNA5
020	ElenasNinethSyntheticRNA_R	aaatatagtttagacaatttgatccagcgatacagcgccagctctgctaagg	part 3 Elenas nineth synthetic shRNA	miRsAg	pC-DNA5
021	ElenasTenthSyntheticRNA_F	attatgtctaaactattaatatcggtgaccgtggtacctagctactgctaggc	part 2 Elenas tenth synthetic shRNA	miRsAg	pC-DNA5
022	ElenasTenthSyntheticRNA_R	attaatagtttagacataatatcggtggccgtggtgtccagctctgctaagg	part 3 Elenas tenth synthetic shRNA	miRsAg	pC-DNA5
023	FADDFWEcoR1	ttttgaattcatgggcggtaggcgtgtacg	FADD
024	gE1A-Fse1_rev	tttttccggccggttatggcctggggcctttaca	E1A rescue gene
025	Hcrfor_HindIII_AgeI_TL	gagtcaagcttaccggttggaggtgaagttaacaccttcgtg	part 1 shRNA against everything; cutting site: HindIII, AgeI	miRsAg	pC-DNA5
026	Hcrrev_ApaI_SalI_TL	ctcgagggcccgtcgacaagcaaacgatgccaagacatttatcg	part 4 shRNA against everything; cutting site: ApaI, SalI	miRsAg	pC-DNA5
027	HSVluc_norm_BglII_fw	tttttagatcttgcaggagcttcagggagtg	HSV-TK, Firefly Luciferase	psiCHECK2	pTR-UF3
028	HSVluc_norm_BglII_rv	ttttagatctggttccgcgcacatttccc	HSV-TK, Firefly Luciferase	psiCHECK2	pTR-UF3
029	Kif11_PLK1_shRNA-hcrfor:ecoRI	gagtcgaattctggaggtgaagttaacaccttcgtg	part 1 shRNA against everything; cutting site: ecoRI	miRsAg	pTR-UF3
030	Kif11&PLK1_shRNA_hcrrev:XbaI	ctcgaagatctaagcaaacgatgccaagacatttatcg	part 4 shRNA against everything; cutting site: XbaI	miRsAg	pTR-UF3
031	Kif11_shRNA_forward	actctgtctaaactatgagtacattaaacaattcctattagctactgctaggc	part 2 shRNA against Kif11	miRsAg	pTR-UF3
032	Kif11_shRNA_reverse	actcatagtttagacagagtacattaatcaattccattcagctctgctaagg	part3 shRNA against Kif11	miRsAg	pTR-UF3
033	PLK1_shRNA_forward	atgatgtctaaactattcattaagcagatcgttaaccgtagctactgctaggc	part2 shRNA against PLK1	miRsAg	pTR-UF3
034	PLK1_shRNA_reverse	atgaatagtttagacatcattaagcagctcgttaatggcagctctgctaagg	part3 shRNA against PLK1	miRsAg	pTR-UF3
035	pTR-UF3_Nsp1_for	ctattacgccagctggatgcatcccagctgc	for pTR-UF3 backbone Pst1->Nsi1 mutation
036	pTR-UF3_Nsp1_rev	gcagctgggatgcatccagctggcgtaatag	for pTR-UF3 backbone Pst1->Nsi1 mutation
037	pTR-UF3_PstI-NsiI_for	gctattacgccagctggatgcatcccagctgcattaatgaatcgg	mutagenesis of PstI to NsiI	pTR-UF3	pTR-UF3
038	pTR-UF3_PstI-NsiI_rev	ccgattcattaatgcagctgggatgcatccagctggcgtaatagc	mutagenesis of PstI to NsiI	pTR-UF3	pTR-UF3
039	pTR-UF3_PstI-NsiI_shifted_rev	cattaatgcagctgggatgcatccagctggcgtaatagcgaagag	mutagenesis of PstI to NsiI	pTR-UF3	pTR-UF3
040	pTRUF3_seq_fwrv	caactccatcactaggggttcct	sequencing purpose	pTR-UF3	pTR-UF3
041	SCLpsv40_FW	ctcaattagtcagcaaccatagtccc	FWprimer for testing stable integration of shRNA into T-Rex		T-REx cells
042	SCLpsv40_FW	gcaaagtgccgataaacataacgatct	RVprimer for testing stable integration of shRNA into T-Rex		T-REx cells
043	Sequencing primer_pTR-UF3 mutated	ctaaatcggaaccctaaagggagcccccg	sequencing of mutagenesis of PstI to NsiI	pTR-UF3	pTR-UF3
044	Sv40lucmcs_AgeI_fw	ttttaccggtgtggaatgtgtgtcagttag	SV40, Renilla Luciferase, MCS	psiCHECK2	pTR-UF3
045	Sv40lucmcs_BglII_rv	tattagatctccgcgtcagacaaaccctaac	SV40, Renilla Luciferase, MCS	psiCHECK2	pTR-UF3
046	tBidGFPRVFse1	tttttggccggccttacatgtacagctcg	tBid death gene	pcherryBidGFP
047	tBidFWEcoR1	tttttgaattcaaccgcagcagccactc	tBid death gene	pcherryBidGFP
048	tBidRVFse1	tttttccggccgggtccatcccatttctg	tBid death gene	pcherryBidGFP
049	TetO2FW	ggggacaacttttctatacaaagttgtccctatcagtgatagagatctc	TetO8	synthesized tetO8	Gateway entry vector

Standart Kit Cloning Primers

ID	Name	Sequence (5' to 3')	amplified	origin	for backbone
D001	backbone_AvrII_fw	ttttcctaggtagcggcgcattaagcgcgg	-	-	-
D002	backbone_AvrII_rev	ttttcctaggcttgttgtagcttaaattttg	-	-	-
D003	backbone_ClaI_fw	ttttatcgattagcggcgcattaagcgcgg	-	-	-
D004	backbone_XhoI_rev	ttttctcgagcttgttgtagcttaaattttg	-	-	-
D005	BBB_Oligo_XhoI	ccggActgcagcggccgcgcgctagcacactagtgcggccgcgaattCa	-	-	-
D006	BBB_Oligo_XmaI	tcgatgaattcgcggccgcactagtgtgctagcgcgcggccgctgcagt	-	-	-
D007	BBB_Oligo_XmaI	tcgatgaattcgcggccgcactagtgtgctagcgcgcggccgctgcagt	-	-	-
D008	BBB_suffix_reverse	aaaactgcagcggccgcgc	-	-	-
D009	BGH_pA_BamHI_rc_fw	ttttgaattcgcggccgcactagtggatccccatagagcccaccgcatccc	-	-	-
D010	BGH_pA_fw	ttttgaattcgcggccgcactagttttaaacccgctgatcagcc	-	-	-
D011	BGH_pA_rev	ttttctgcagcggccgcgctagcccatagagcccaccgcatccc	-	-	-
D012	BGH_pA_rc_rev	ttttctgcagcggccgcgctagctttaaacccgctgatcagcc	-	-	-
D013	biCMV_BBB_fw	ttttgaattcgcggccgcactagtgcgatctgacggttcactaaacg	-	-	-
D014	biCMV_BBB_fw	ttttgaattcgcggccgcactagtgcgatctgacggttcactaaacg	-	-	-
D015	biCMV_BBB_rev	ttttctgcagcggccgcgcgctagcacagcggatctgacggttcac	-	-	-
D016	biCMV_rc_BBB_fw	ttttgaattcgcggccgcactagtagcggatctgacggttcac	-	-	-
D017	biCMV_rc_BBB_rev	ttttctgcagcggccgcgctagcgcgatctgacggttcac	-	-	-
D018	CMV_BBB_BamHI_fw	gaattcgcggccgcactagtggatcccgatgtacgggccagatatacg	-	-	-
D019	CMV_BBB_fw	ttttgaattcgcggccgcactagtcgatgtacgggccagatatacg	-	-	-
D020	CMV_BBB_rev	ttttctgcagcggccgcgcgctagcacatttcgataagccagtaagc	-	-	-
D021	CMV_Teto2_BBB_fw	tttt gaattcgcggccgcactagtgttgacattgattattgtctag	-	-	-
D022	CMV_TetO2_BBB_rev	tttt ctgcagcggccgcgcgctagcaccggaggctggatcggtc	-	-	-
D023	CMV_rc_BBB_fw	ttttgaattcgcggccgcactagtatttcgataagccagtaagc	-	-	-
D024	CMV_rc_BBB_rev	ttttctgcagcggccgcgctagccgatgtacgggccagatatacg	-	-	-
D025	FRT_rev	ctgcagcggccgcgctagcccaaggaagttcctatactttc	-	-	-
D026	FRT_rev	gaattcgcggccgcactagtttctcgccacgttcgccggc	-	-	-
D027	hRluc_ter_BBB_fw	ttttgaattcgcggccgcactagtatggcttccaaggtgtacgac	-	-	-
D028	hRluc_ter_BBB_rev	ttttctgcagcggccgcgctagcttactgctcgttcttcagcacgc	-	-	-
D029	hsa-mir-122_BBB(perf)	ttttgaattcgcggccgcactagtcaaacaccattgtcacactccagctagcgcggccgctgcagtttt	-	-	-
D030	hsa-mir-122_BBB(rand9-12)	ttttgaattcgcggccgcactagtcaaacaccatnnnnacactccagctagcgcggccgctgcagtttt	-	-	-
D031	hsa-mir-122_BBB(rand9-22)	ttttgaattcgcggccgcactagtnnnnnnnnnnnnnnacactccagctagcgcggccgctgcagtttt	-	-	-
D032	hsa-mir-122_Sgf_Not(perf)	ttttgcgatcgccaaacaccattgtcacactccagcggccgctatgtcacaact	-	-	-
D033	hsa-mir-122_Sgf_Not(rand9-12)	ttttgcgatcgccaaacaccatnnnnacactccagcggccgctatgtcacaact	-	-	-
D034	hsa-mir-122_Sgf_Not(rand9-22)	ttttgcgatcgcnnnnnnnnnnnnnnacactccagcggccgctatgtcacaact	-	-	-
D035	hsa-mir-375_BBB(perf)	ttttgaattcgcggccgcactagttcacgcgagccgaacgaacaaagctagcgcggccgctgcagtttt	-	-	-
D036	hsa-mir-375_BBB(rand9-12)	ttttgaattcgcggccgcactagttcacgcgagcnnnncgaacaaagctagcgcggccgctgcagtttt	-	-	-
D037	hsa-mir-375_BBB(rand9-22)	ttttgaattcgcggccgcactagtnnnnnnnnnnnnnncgaacaaagctagcgcggccgctgcagtttt	-	-	-
D038	hsa-mir-375_Sgf_Not(perf)	ttttgcgatcgctcacgcgagccgaacgaacaaagcggccgctatgtcacaact	-	-	-
D039	hsa-mir-375_Sgf_Not(rand9-12)	ttttgcgatcgctcacgcgagcnnnncgaacaaagcggccgctatgtcacaact	-	-	-
D040	hsa-mir-375_Sgf_Not(rand9-22)	ttttgcgatcgcnnnnnnnnnnnnnncgaacaaagcggccgctatgtcacaact	-	-	-
D041	hsa-mir-376a_BBB(perf)	ttttgaattcgcggccgcactagtacgtggattttcctctatgatgctagcgcggccgctgcagtttt	-	-	-
D042	hsa-mir-376a_BBB(rand9-12)	ttttgaattcgcggccgcactagtacgtggattnnnntctatgatgctagcgcggccgctgcagtttt	-	-	-
D043	hsa-mir-376a_BBB(rand9-22)	ttttgaattcgcggccgcactagtnnnnnnnnnnnnntctatgatgctagcgcggccgctgcagtttt	-	-	-
D044	hsa-mir-376a_Sgf_Not(perf)	ttttgcgatcgcacgtggattttcctctatgatgcggccgctatgtcacaact	-	-	-
D045	hsa-mir-376a_Sgf_Not(rand9-12)	ttttgcgatcgcacgtggattnnnntctatgatgcggccgctatgtcacaact	-	-	-
D046	hsa-mir-376a_Sgf_Not(rand9-22)	ttttgcgatcgcnnnnnnnnnnnnntctatgatgcggccgctatgtcacaact	-	-	-
D047	igem20100723_01	tgccacctgacgtctaagaa	-	-	-
D048	igem20100723_02	attaccgcctttgagtgagc	-	-	-
D049	Luc2_ter_BamHI_BBB_rev	ttttctgcagcggccgcgctagcggatcctaccacatttgtagaggttttac	-	-	-
D050	Luc2_ter_BamHI_BBB_rev	ttttctgcagcggccgcgctagcggatcctaccacatttgtagaggttttac	-	-	-
D051	Luc2_ter_BBB_fw	ttttgaattcgcggccgcactagtgccaccatggaagatgcc	-	-	-
D052	mir122_miMeasure_screen_fev	ccgcgctagctggagtgt	-	-	-
D053	Oligo_Xba_mut_fw	ctagcctcgagttctgaccgccccgggg	-	-	-
D054	Oligo_Xba_mut_rev	ctagccccggggcggtcagaactcgagg	-	-	-
D055	pSMB_miMeasure_Screen_fw	gcacaagctggagtacaactac	-	-	-
D056	pSMB_miMeasure_Screen_rev	ggtttcccgactggaaagcg	-	-	-
D057	RSV_fw	ttttgaattcgcggccgcactagtcaattctcatgtttgacagc	-	-	-
D058	RSV_rev	ttttctgcagcggccgcgctagccagcttggaggtgcacacc	-	-	-
D059	RSV_rc_fw	ttttgaattcgcggccgcactagtcagcttggaggtgcacacc	-	-	-
D060	RSV_rc_rev	ttttctgcagcggccgcgctagccaattctcatgtttgacagc	-	-	-
D061	RSV_fw_midcomp	cgaaccactgaataccgcattgcag	-	-	-
D062	RSV_rev_midcomp	ctgcaatgcggtattcagtggttcg	-	-	-
D063	SB-prep-3P	gccgctgcagtccggcaaaaaaacg	-	-	-
D064	SB-prep-2Ea	atgaattccagaaatcatccttagcg	-	-	-
D065	second_strand_notI	agttgtgacatagcggccgc	-	-	-
D066	shRNA-6th-bs(custom)_BBB	ttttgaattcgcggccgcactagtnnnnnnnnnnnnnncgtcaatagctagcgcggccgctgcagtttt	-	-	-
D067	shRNA-7th-bs(custom)_BBB	ttttgaattcgcggccgcactagtnnnnnnnnnnnnnntcaatagagctagcgcggccgctgcagtttt	-	-	-
D068	shRNA-8th-bs(custom)_BBB	ttttgaattcgcggccgcactagtnnnnnnnnnnnnnngctgatgtgctagcgcggccgctgcagtttt	-	-	-
D069	shRNA-9th-bs(custom)_BBB	ttttgaattcgcggccgcactagtnnnnnnnnnnnnnnctagtttagctagcgcggccgctgcagtttt	-	-	-
D070	shRNA-10th-bs(custom)_BBB	ttttgaattcgcggccgcactagtnnnnnnnnnnnnnngctataatgctagcgcggccgctgcagtttt	-	-	-
D071	shRNA-6th-bs(perf)_BBB	ttttgaattcgcggccgcactagtcgcaacctcactgccgtcaatagctagcgcggccgctgcagtttt	-	-	-
D072	shRNA-7th-bs(perf)_BBB	ttttgaattcgcggccgcactagtcaacctcactgccgtcaatagagctagcgcggccgctgcagtttt	-	-	-
D073	shRNA-8th-bs(perf)_BBB	ttttgaattcgcggccgcactagtcaacgacgtatttggctgatgtgctagcgcggccgctgcagtttt	-	-	-
D074	shRNA-9th-bs(perf)_BBB	ttttgaattcgcggccgcactagtcgcgacatagcgacctagtttagctagcgcggccgctgcagtttt	-	-	-
D075	shRNA-10th-bs(perf)_BBB	ttttgaattcgcggccgcactagtctgtggtgccggtggctataatgctagcgcggccgctgcagtttt	-	-	-
D076	shRNA-6th-bs(rand9-12)_BBB	gcgatcgccgcaacctcannnncgtcaatagcggccgctatgtcacaact	-	-	-
D077	shRNA-7th-bs(rand9-12)_BBB	ttttgaattcgcggccgcactagtcaacctcactnnnntcaatagagctagcgcggccgctgcagtttt	-	-	-
D078	shRNA-8th-bs(rand9-12)_BBB	ttttgaattcgcggccgcactagtcaacgacgtannnngctgatgtgctagcgcggccgctgcagtttt	-	-	-
D079	shRNA-9th-bs(rand9-12)_BBB	ttttgaattcgcggccgcactagtcgcgacatagnnnnctagtttagctagcgcggccgctgcagtttt	-	-	-
D080	shRNA-10th-bs(rand9-12)_BBB	ttttgaattcgcggccgcactagtctgtggtgccnnnngctataatgctagcgcggccgctgcagtttt	-	-	-
D081	shRNA-6th-bs(custom)_SgfI_NotI	gcgatcgcnnnnnnnnnnnnnncgtcaatagcggccgctatgtcacaact	-	-	-
D082	shRNA-7th-bs(custom)_SgfI_NotI	gcgatcgcnnnnnnnnnnnnnntcaatagagcggccgctatgtcacaact	-	-	-
D083	shRNA-8th-bs(custom)_SgfI_NotI	gcgatcgcnnnnnnnnnnnnnngctgatgtgcggccgctatgtcacaact	-	-	-
D084	shRNA-9th-bs(custom)_SgfI_NotI	gcgatcgcnnnnnnnnnnnnnnctagtttagcggccgctatgtcacaact	-	-	-
D085	shRNA-10th-bs(custom)_SgfI_NotI	gcgatcgcnnnnnnnnnnnnnngctataatgcggccgctatgtcacaact	-	-	-
D086	shRNA-6th-bs(perf)_SgfI_NotI	gcgatcgccgcaacctcactgccgtcaatagcggccgctatgtcacaact	-	-	-
D087	shRNA-7th-bs(perf)_SgfI_NotI	gcgatcgccaacctcactgccgtcaatagagcggccgctatgtcacaact	-	-	-
D088	shRNA-8th-bs(perf)_SgfI_NotI	gcgatcgccaacgacgtatttggctgatgtgcggccgctatgtcacaact	-	-	-
D089	shRNA-9th-bs(perf)_SgfI_NotI	gcgatcgccgcgacatagcgacctagtttagcggccgctatgtcacaact	-	-	-
D090	shRNA-10th-bs(perf)_SgfI_NotI	gcgatcgcctgtggtgccggtggctataatgcggccgctatgtcacaact	-	-	-
D091	shRNA-6th-bs(rand9-12)_SgfI_NotI	gcgatcgccgcaacctcannnncgtcaatagcggccgctatgtcacaact	-	-	-
D092	shRNA-7th-bs(rand9-12)_SgfI_NotI	gcgatcgccaacctcactnnnntcaatagagcggccgctatgtcacaact	-	-	-
D093	shRNA-8th-bs(rand9-12)_SgfI_NotI	gcgatcgccaacgacgtannnngctgatgtgcggccgctatgtcacaact	-	-	-
D094	shRNA-9th-bs(rand9-12)_SgfI_NotI	gcgatcgccgcgacatagnnnnctagtttagcggccgctatgtcacaact	-	-	-
D095	shRNA-10th-bs(rand9-12)_SgfI_NotI	gcgatcgcctgtggtgccnnnngctataatgcggccgctatgtcacaact	-	-	-
D096	shRNA_AflII_BBB_fw	ttttctgcagcggccgcgcgctagccttaagtggaggtgaagttaacaccttcgtg	-	-	-
D097	shRNA_HindIII_BBB_rev	ttttgaattcgcggccgcactagtaagcttaagcaaacgatgccaagacatttatcg	-	-	-
D098	SV40_BamHI_BBB_fw	ctgcagcggccgcgctagcggatccaagctttttgcaaaagcctagg	-	-	-
D099	SV40_BBB_rev	ttttctgcagcggccgcgctagcaagctttttgcaaaagcctaggc	-	-	-
D100	SV40_rc_BBB_fw	ttttgaattcgcggccgcactagtaagctttttgcaaaagcc	-	-	-
D101	SV40_rc_BBB_rev	ttttctgcagcggccgcgctagcgcgcagcaccatggcctg	-	-	-
D102	SV40_term_fw	ttttgaattcgcggccgcactagtcagacatgataagatacattg	-	-	-
D103	SV40_Xho_Xma_BBB_fw	gaattcgcggccgcactagtctcgagtttctcccggggcgcagcaccatggcctg	-	-	-
D104	TetRepressor (from pcDNA6)fw	ttttgaattcgcggccgcactagtgccaccatgtctagattagataaaag	-	-	-
D105	TetRepressor (from pcDNA6)rew	ttttctgcagcggccgcgctagcttaataagatctaaattcccgcgatccgc	-	-	-

ViroBytes primers

ID	Name	Sequence (5' to 3')	amplified fragment	direction	cap gene of AAV
R001	Anchor12367891012	tctctctctctctctaagcttgtctgagtgactagcattcgttaattaacTACCG	anchor	-
R002	Anchor4	tctctctctctctctaagcttgtctgagtgactagcattcgttaattaactactg	anchor	-
R003	Anchor5	tctctctctctctctaagcttgtctgagtgactagcattcgttaattaactacag	anchor	-
R004	Anchor_comp_all	gttaattaacgaatgctagtcactcagacaagctt	anchor	-
R005	F1_for_cap12678910	gctacggtctcaatggctgccgatggttatcttccag	1	forward	1,2,6,7,8,9,10
R006	F1_for_cap5	gctacggtctcaatggctgccgatggttatcttccag	1	forward	5
R007	F1_rev_cap2	gctacggtctcgctcctgaaactcggcgtcggcgtgg	1	reverse	2
R008	F1_rev_cap5	gctacggtctctctcctgaaactcggcgtccgcgtgg	1	reverse	5
R009	F1_rev_cap9	gctacggtctcgctcctggaactcggcgtcggcgtgg	1	reverse	9
R010	F1_rev_cap168	gctacggtctcgctcctgaaactcggcgtcggcgtgg	1	reverse	1,6,8
R011	F2_for_cap2	gctacggtctctggagcgccttaaagaagatacgtcttttggggg	2	forward	2
R012	F2_for_cap5	gctacggtctctggagaagctcgccgacgacacatccttcggggg	2	forward	5
R013	F2_for_cap9	gctacggtctctggagcggctcaaagaagatacgtcttttggggg	2	forward	9
R014	F2_for_cap168	gctacggtctctggagcgtctgcaagaagatacgtcttttggggg	2	forward	1,6,8
R015	F2_rev_cap2	gctacggtctcgtgtcgcccatccatgtggaatcgcaatgc	2	reverse	2
R016	F2_rev_cap5	gctacggtctcgtgtcccccatccacgtggaatcgcaatgc	2	reverse	5
R017	F2_rev_cap9	gctacggtctcgtgtcccccagccattgggaatcgcaatgc	2	reverse	9
R018	F2_rev_cap168	gctacggtctcgtgtcgcccagccatgtggaatcgcaatgc	2	reverse	1,6,8
R019	F3_for_cap5	gctacggtctccgacagagtcatcaccaccagcacccg	3	forward	5
R020	F3_for_cap9	gctacggtctccgacagagtcatcaccaccagcacccg	3	forward	9
R021	F3_for_cap1268	gctacggtctccgacagagtcatcaccaccagcacccg	3	forward	1,2,6,8
R022	F3_rev_cap1	gctacggtctcaggttattagcgatggttgtgacgccatcattcgtc	3	reverse	1
R023	F3_rev_cap2	gctacggtctcaggttattggcaatcgtcgtcgtaccgtcattctgc	3	reverse	2
R024	F3_rev_cap5	gctacggtctcaggttgttggcgatggtggtggtggagtcctgcac	3	reverse	5
R025	F3_rev_cap6	gctacggtctcaggttattagcgatggtcgtgacgccatcattcgtc	3	reverse	6
R026	F3_rev_cap8	gctacggtctcaggttattggcgatggtcttggtgccttcattctgc	3	reverse	8
R027	F3_rev_cap9	gctacggtctcaggttattggcgatggtcttgactccattgttgtcc	3	reverse	9
R028	F4_for_cap2	gctacggtctctaaccttaccagcacggttcaggtgtttactgactc	4	forward	2
R029	F4_for_cap5	gctacggtctccaacctcacctccaccgtccaagtgtttacggacga	4	forward	5
R030	F4_for_cap8	gctacggtctctaacctcaccagcaccatccaggtgtttacggactc	4	forward	8
R031	F4_for_cap9	gctacggtctctaaccttaccagcacggtccaggtcttcacggactc	4	forward	9
R032	F4_for_cap16	gctacggtctctaaccttaccagcacggttcaagtcttctcggactc	4	forward	1,6
R033	F4_reverse_cap1	gctacggtctcagctgctgtggaaaggcacttcctcaaaggtg	4	reverse	1
R034	F4_reverse_cap2	gctacggtctcagctgctgtggaaaggaacgtcctcaaaagtg	4	reverse	2
R035	F4_reverse_cap5	gctacggtctcagctggagtggaagggcacctcctcaaagttg	4	reverse	5
R036	F4_reverse_cap9	gctacggtctcagctgctatggaaaggtacgttctcaaactcg	4	reverse	9
R037	F4_reverse_cap68	gctacggtctcagctgctgtggaaaggcacgtcctcgaaggtg	4	reverse	6,8
R038	F5_for_cap2	gctacggtctcgcagctacgctcacagccagagtctggaccgtc	5	forward	2
R039	F5_for_cap5	gctacggtctcccagcttcgctcccagtcagaacctgttcaagc	5	forward	5
R040	F5_for_cap8	gctacggtctcgcagctacgcccacagccagagcttggaccggc	5	forward	8
R041	F5_for_cap9	gctacggtctcgcagctacgctcacagccaaagcctggaccgac	5	forward	9
R042	F5_for_cap16	gctacggtctcgcagctacgcgcacagccagagcctggaccggc	5	forward	1,6
R043	F5_rev_cap2	gctacggtctccagttcctagactggtcccgaatgtcactcg	5	reverse	2
R044	F5_rev_cap5	gctacggtctccagtttttgtaggtgttggcgtatctcccgg	5	reverse	5
R045	F5_rev_cap8	gctacggtctccagttctttgcctgattggccattgtattag	5	reverse	8
R046	F5_rev_cap9	gctacggtctctagtttcttccctggacagccatgttgctgg	5	reverse	9
R047	F5_rev_cap16	gctacggtctccagtttttgggctgaacagacatgccagctg	5	reverse	1,6
R048	F6_for_cap2	gctacggtctcgaactggcttcctggaccctgttaccgccagc	6	forward	2
R049	F6_for_cap5	gctacggtctcaaactggttcccggggcccatgggccgaaccc	6	forward	5
R050	F6_for_cap8	gctacggtctcgaactggctgccaggaccctgttaccgccaac	6	forward	8
R051	F6_for_cap9	gctacggtctcaaactacatacctggacccagctaccgacaac	6	forward	9
R052	F6_for_cap16	gctacggtctcaaactggctacctggaccctgttatcggcagc	6	forward	1,6
R053	F6_rev_cap1	gctacggtctcgccacagggttagtggctttaatttcctcttcgtctg	6	reverse	1
R054	F6_rev_cap2	gctacggtctcgccacgggattggttgtcctgatttcctcttcgtctg	6	reverse	2
R055	F6_rev_cap5	gctacggtctcgccacgcggttcaccggctgcgtctcgctctcgctgg	6	reverse	5
R056	F6_rev_cap6	gctacggtctcgccacggggttagtggctttgatttcctcttcgtctg	6	reverse	6
R057	F6_rev_cap8	gctacggtctcgccacagggttagtggttttgatttcttctcgctgg	6	reverse	8
R058	F6_rev_cap9	gctacggtctcgccaccgggttagtagttttaatttcttcttcgttggttatc	6	reverse	9
R059	F7_for_cap1	gctacggtctctgtggccaccgaaagatttgggaccgtggcagtcaatttc	7	forward	1
R060	F7_for_cap2	gctacggtctccgtggctacggagcagtatggttctgtatctaccaacctc	7	forward	2
R061	F7_for_cap5	gctacggtctccgtggcgtacaacgtcggcgggcagatg	7	forward	5
R062	F7_for_cap6	gctacggtctccgtggccaccgaaagatttgggactgtggcagtcaatctc	7	forward	6
R063	F7_for_cap8	gctacggtctctgtggctacagaggaatacggtatcgtggcagataacttg	7	forward	8
R064	F7_for_cap9	gctacggtctcggtagcaacggagtcctatggacaagtggccacaaaccac	7	forward	9
R065	F7_rev_cap1	gctacggtctcggtgatgaatgaagcaaactttgtagctgaaaac	7	reverse	1
R066	F7_rev_cap2	gctacggtctctgtgatgaaggaagcaaactttgccgcactgaag	7	reverse	2
R067	F7_rev_cap5	gctacggtctcggtgatgaagctgctgacgggcacgtccgagaag	7	reverse	5
R068	F7_rev_cap6	gctacggtctcggtgatgaatgaagcaaactttgtagccgaaaac	7	reverse	6
R069	F7_rev_cap8	gctacggtctccgtgatgaaagagttcagctttgactggttgaag	7	reverse	8
R070	F7_rev_cap9	gctacggtctcggtgatgaaagagttcagcttgtccttgttgaag	7	reverse	9
R071	F8_for_cap1	gctacggtctcatcacccaatactccacaggacaagtgagtgtgg	8	forward	1
R072	F8_for_cap2	gctacggtctcatcacacagtactccacgggacaggtcagcgtgg	8	forward	2
R073	F8_for_cap5	gctacggtctcatcacccagtacagcaccgggcaggtcaccgtgg	8	forward	5
R074	F8_for_cap6	gctacggtctcatcacccagtattccacaggacaagtgagcgtgg	8	forward	6
R075	F8_for_cap8	gctacggtctcatcacgcaatacagcaccggacaggtcagcgtgg	8	forward	8
R076	F8_for_cap9	gctacggtctcatcacccagtattctactggccaagtcagcgtgg	8	forward	9
R077	F8_rev_cap1	gctacggcgcgccttacaggggacgggtaaggtaacgggtgcc	8	reverse	1
R078	F8_rev_cap2	gctacggcgcgccttacagattacgagtcaggtatctggtgccaatggg	8	reverse	2
R079	F8_rev_cap5	gctacggcgcgccttaaaggggtcgggtaaggtatcgggttccgatagg	8	reverse	5
R080	F8_rev_cap6	gctacggcgcgccttacaggggacgggtgaggtaacgggtgcc	8	reverse	6
R081	F8_rev_cap8	gctacggcgcgccttacagattacgggtgaggtaacgggtgccaatggg	8	reverse	8
R082	F8_rev_cap9	gctacggcgcgccttacagattacgagtcaggtatctggtgccaatggg	8	reverse	9
R083	Virbyte_for	gtctgagtgactagcattcggtctgagtgactagcattcg	-	forward
R084	Virobyte_rev	gctacggcgcgcctta	-	reverse

Primer Table Quick 'n' Dirty

ID	Name	Sequence (5' to 3')	amplified	origin	for backbone
L001	CMV_AgeI_fw	ttttaccggtagaatctgcttagggttagg	for use with L11	from pcDNA5+miRNA	to tuning construct-pTR-UF3 KpnI/SalI
L002	CMVshRNApA_KpnI_rv	taatggtacccagaagccatagagcccacc	-	from pcDNA5+miRNA	to tuning construct-pTR-UF3 KpnI/SalI
L003	HSVluc_norm_BglII_fw	tttttagatcttgcaggagcttcagggagtg	for use with L8	from PsiCheck2	to pTR-UF3 BglII
L004	HSVluc_norm_BglII_rv	ttttagatctggttccgcgcacatttccc	for use with L7	from PsiCheck2	to pTR-UF3 BglII
L005	HSVluc_norm_KpnI_fw	tttttggtagcaggagcttcagggagtg	for use with L16	from PsiCheck2	to pTR-UF3 KpnI/SalI
L006	HSVluc_norm_SalI_rv	ttttgtcgacggttccgcgcacatttccc	for use with L15	from PsiCheck2	to pTR-UF3 KpnI/SalI
L007	Sv40lucmcs_AgeI_fw	ttttaccggtgtggaatgtgtgtcagttag	for use with L13	from PsiCheck2	to tuning construct-pTR-UF3 KpnI/SalI
L008	SV40luc_SalI_rv	tattgtcgacccgcgtcagacaaaccctaac	for use with L1	from PsiCheck2	to tuning construct-pTR-UF3 KpnI/SalI
L009	TetO2_StuI_NheI	ttttaggccttccctatcagtgatagagatctccctatcagtgatagagagctagctaac	Tet Repressor	Oligo	to final tuning construct
L010	TetR_BspEI_fw	tttttccggaggcgaattgatatgtctagattag	for use with L14	from pcDNA6/TR	pTR-UF3 BspEI/NotI
L011	TetR_SgfI_NotI_rv	ttttgcggccgctggcgatcgctaataagatctgaattcccgggatc	for use with L12	from pcDNA6/TR	to pTR-UF3 BspEI/NotI

Primer Table raPCR

ID	Name	Sequence (5' to 3')	target	Assembly Site
ra001	psicheck2_MCS_min_200_fw	cagcgacgatctgcctaa	sequencing fw		sequencing Primer for binding-sites in pSiCheck vector
ra002	psicheck2_MCS_plu_200_rv	gtggccaccaagaccaaa	sequencing rv		sequencing Primer for binding-sites in pSiCheck vector
ra003	raPCR_AS13-hsa-mir-122	cactgaatccaactgcaaacaccattgtcacactccagcatacatggactgc	hsa-mir-122	13 bp
ra004	raPCR_AS13-has-mir122(ran9-12)	cactgaatccaactgcaaacaccatnnnnacactccagcatacatggactgc	has-mir122 (ran9-12)	13 bp	randomised nucleotides 9-12
ra005	raPCR_AS13-hsa-mir-320b	cactgaatccaactgttgccctctcaacccagcttttgcatacatggactgc	hsa-mir-320b	13 bp
ra006	raPCR_AS13-has-mir-221	cactgaatccaactggaaacccagcagacaatgtagctgcatacatggactgc	has-mir-221	13 bp
ra007	raPCR_AS13-has-mir-221(ran9-12	cactgaatccaactggaaacccagcannnnatgtagctgcatacatggactgc	has-mir-221 (ran9-12)	13 bp	randomised nucleotides 9-12
ra008	raPCR_AS13-has-mir-1179	cactgaatccaactgccaaccaatgaaagaatgcttgcatacatggactgc	has-mir-1179	13 bp
ra009	raPCR_AS13-has-mir-1179ran9-12	cactgaatccaactgccaaccaatnnnngaatgcttgcatacatggactgc	has-mir-1179 (ran9-12)	13 bp	randomised nucleotides 9-12
ra010	raPCR_AS13-has-mir4286	cactgaatccaactgggtaccaggagtggggtgcatacatggactgc	has-mir4286	13 bp
ra011	raPCR_AS13-mm-mir-375	cactgaatccaactgtcacgcgagccgaacgaacaaagcatacatggactgc	mm-mir-375	13 bp
ra012	raPCR_AS13-mm-mir-375(ran9-12)	cactgaatccaactgtcacgcgagcnnnncgaacaaagcatacatggactgc	mm-mir-375 (ran9-12)	13 bp	randomised nucleotides 9-12
ra013	raPCR_AS13-mm-mir-376a	cactgaatccaactgacgtggattttcctctacgatgcatacatggactgc	mm-mir-376a	13 bp
ra014	raPCR_AS13-spacer(0)	cagttggattcagtggcagtccatgtatgc	spacer	13 bp
ra015	raPCR_AS13-spacer(10)	cagttggattcagtggctatttctcgcagtccatgtatgc	spacer	13 bp
ra016	raPCR_AS13-spacer(20)	cagttggattcagtgatgacaggtagctatttctcgcagtccatgtatgc	spacer	13 bp
ra017	raPCR_AS13-stop_fw_BBB	ttttgaattcgcggccgcactagtcactgaatccaactg	stop fw	13 bp
ra018	raPCR_AS13-stop_rev_BBB	ttttctgcagcggccgcgctagcgcagtccatgtatgc	stop rev	13 bp
ra019	raPCR_AS13-stop_rev_NotI	ttttgcggccgctggagtgtgacaatggtgtttg	stop rev	13 bp
ra020	raPCR_AS13-stop_fw_XhoI	ttttctcgagcactgaatccaactg	stop fw	13 bp

mouse oligos

ID	Name	Sequence (5' to 3')
mouse1	CMV_TetR_122bs_SalI_fw	ttttgtcgaccgttgacattgattattgac
mouae2	CMV_TetR_both_MluI_rev	ttttacgcgttaacacacaaaaaaccaacac
mouse3	CMV_TetR_control_SalI_fw	ttttgtcgaccgttgacattgattattgac
mouse4	CMV_TetO2_EcoRI_fw	ttttgaattcgttgacattgattattgtctag
mouse5	Luc2_Ter_PstI_rev	ttttctgcagtaccacatttgtagaggttttac
mouse6XXX	haat_bs_p_fw	tcgaggaagcgtttaggcatgtttaacatctctagagc
mouse7	haat_bs_p_rev	ggccgctctagagatgttaaacatgcctaaacgcttcc
mouse8	haat_bs_r10_12_aat_fw	tcgaggaagcgtaatggcatgtttaacatctctagagc
mouse9	haat_bs_r10_12_aat_rev	ggccgctctagagatgttaaacatgccattacgcttcc
mouse10	haat_bs_r10_12_agc_fw	tcgaggaagcgtagcggcatgtttaacatctctagagc
mouse11	haat_bs_r10_12_agc_rev	ggccgctctagagatgttaaacatgccgctacgcttcc
mouse12	haat_bs_m10at_fw	tcgaggaagcgttttggcatgtttaacatctctagagc
mouse13	haat_bs_m10at_rev	ggccgctctagagatgttaaacatgccaaaacgcttcc
mouse14	haat_bs_m10ac_fw	tcgaggaagcgtttcggcatgtttaacatctctagagc
mouse15	haat_bs_m10ac_rev	ggccgctctagagatgttaaacatgccgaaacgcttcc
mouse16	haat_bs_m11ta_fw	tcgaggaagcgttaaggcatgtttaacatctctagagc
mouse17	haat_bs_m11ta_rev	ggccgctctagagatgttaaacatgccttaacgcttcc
mouse18	haat_bs_m11tg_fw	tcgaggaagcgttgaggcatgtttaacatctctagagc
mouse19	haat_bs_m11tg_rev	ggccgctctagagatgttaaacatgcctcaacgcttcc
mouse20	haat_bs_r9_12_aatc_fw	tcgaggaagcgtaatcgcatgtttaacatctctagagc
mouse21	haat_bs_r9_12_aatc_rev	ggccgctctagagatgttaaacatgcgattacgcttcc
mouse22	haat_bs_r9_12_agcc_fw	tcgaggaagcgtagccgcatgtttaacatctctagagc
mouse23	haat_bs_r9_12_agcc_rev	ggccgctctagagatgttaaacatgcggctacgcttcc
mouse24	haat_bs_onlyseed_fw	tcgagcttcgcaaatcgcatgtttaacatctctagagc
mouse25	haat_bs_onlyseed_rev	ggccgctctagagatgttaaacatgcgatttgcgaagc
mouse26	haat_bs_r16-18_fw	tcgaggttccgtttaggcatgtttaacatctctagagc
mouse27	haat_bs_r16-18_rev	ggccgctctagagatgttaaacatgcctaaacggaacc
mouse28XXX	sAg_bs_p_fw	tcgagctcagtttactagtgccatttgttctctagagc
mouse29	sAg_bs_p_rev	ggccgctctagagaacaaatggcactagtaaactgagc
mouse30	sAg_bs_r10_12_acg_fw	tcgagctcagtttactagacgcatttgttctctagagc
mouse31	sAg_bs_r10_12_acg_rev	ggccgctctagagaacaaatgcgtctagtaaactgagc
mouse32	sAg_bs_r10_12_taa_fw	tcgagctcagtttactagtaacatttgttctctagagc
mouse33	sAg_bs_r10_12_taa_rev	ggccgctctagagaacaaatgttactagtaaactgagc
mouse34	sAg_bs_r9_12_acgg_fw	tcgagctcagtttactagacggatttgttctctagagc
mouse35	sAg_bs_r9_12_acgg_rev	ggccgctctagagaacaaatccgtctagtaaactgagc
mouse36	sAg_bs_r9_12_atgt_fw	tcgagctcagtttactagatgtatttgttctctagagc
mouse37	sAg_bs_r9_12_atgt_rev	ggccgctctagagaacaaatacatctagtaaactgagc
mouse38	sAg_bs_m10cg_fw	tcgagctcagtttactagtggcatttgttctctagagc
mouse39	sAg_bs_m10cg_rev	ggccgctctagagaacaaatgccactagtaaactgagc
mouse40	sAg_bs_m10ca_fw	tcgagctcagtttactagtgacatttgttctctagagc
mouse41	sAg_bs_m10ca_rev	ggccgctctagagaacaaatgtcactagtaaactgagc
mouse42	sAg_bs_m11gc_fw	tcgagctcagtttactagtcccatttgttctctagagc
mouse43	sAg_bs_m11gc_rev	ggccgctctagagaacaaatgggactagtaaactgagc
mouse44	sAg_bs_m11ga_fw	tcgagctcagtttactagtaccatttgttctctagagc
mouse45	sAg_bs_m11ga_rev	ggccgctctagagaacaaatggtactagtaaactgagc
mouse46	sAg_bs_onlyseed_fw	tcgagctcagtaatgatcacggatttgttctctagagc
mouse47	sAg_bs_onlyseed_rev	ggccgctctagagaacaaatccgtgatcattactgagc
mouse48	sAg_bs_r16-18_fw	tcgagctcagttatgtagtgccatttgttctctagagc
mouse49	sAg_bs_r16-18_rev	ggccgctctagagaacaaatggcactacataactgagc
mouse50XXX	hAAT_cDNA_SalI_Bluescript_fw	ttttgtcgacgaccatgattacgccaagcg
mouse51	hAAT_cDNA_SalI_fw	ttttgtcgacgggtaccgggccccccgtcgaggatg
mouse52	hAAT_cDNA_XhoI_NotI_NheI_rev	ttttgctagcgcggccgcgtatctcgaggctcaacccttctttaatg
mouse53	hAAT_cDNA_sAg_bs_p_NheI_rev	ttttgctagcgaacaaatggcactagtaaactgaggctcaacccttctttaatg
mouse54	hAAT_cDNA_sAg_bs_r10_12_acg_NheI_rev	ttttgctagcgaacaaatgcgtctagtaaactgaggctcaacccttctttaatg
mouse55	hAAT_cDNA_sAg_bs_r10_12_taa_NheI_rev	ttttgctagcgaacaaatgttactagtaaactgaggctcaacccttctttaatg
mouse56	hAAT_cDNA_bs_r9_12_acgg_NheI_rev	ttttgctagcgaacaaatccgtctagtaaactgaggctcaacccttctttaatg
mouse57	hAAT_cDNA_sAg_bs_r9_12_atgt_NheI_rev	ttttgctagcgaacaaatacatctagtaaactgaggctcaacccttctttaatg
mouse58	hAAT_cDNA_sAg_bs_m10cg_NheI_rev	ttttgctagcgaacaaatgccactagtaaactgaggctcaacccttctttaatg
mouse59	hAAT_cDNA_sAg_bs_m10ca_NheI_rev	ttttgctagcgaacaaatgtcactagtaaactgaggctcaacccttctttaatg
mouse60	hAAT_cDNA_sAg_bs_m11gc_NheI_rev	ttttgctagcgaacaaatgggactagtaaactgaggctcaacccttctttaatg
mouse61	hAAT_cDNA_sAg_bs_m11ga_NheI_rev	ttttgctagcgaacaaatggtactagtaaactgaggctcaacccttctttaatg
mouse62	hAAT_cDNA_sAg_bs_onlyseed_NheI_rev	ttttgctagcgaacaaatccgtgatcattactgaggctcaacccttctttaatg
mouse63	hAAT_cDNA_sAg_bs_r16-18_NheI_rev	ttttgctagcgaacaaatggcactacataactgaggctcaacccttctttaatg
mouse64XXX	sAg_bs_p_fw_E	aattcctcagtttactagtgccatttgttcctgca
mouse65	sAg_bs_p_rev_P	ggaacaaatggcactagtaaactgagg
mouse66	sAg_bs_r10_12_acg_fw_E	aattcctcagtttactagacgcatttgttcctgca
mouse67	sAg_bs_r10_12_acg_rev_P	ggaacaaatgcgtctagtaaactgagg
mouse68	sAg_bs_r10_12_taa_fw_E	aattcctcagtttactagtaacatttgttcctgca
mouse69	sAg_bs_r10_12_taa_rev_P	ggaacaaatgttactagtaaactgagg
mouse70	sAg_bs_r9_12_acgg_fw_E	aattcctcagtttactagacggatttgttcctgca
mouse71	sAg_bs_r9_12_acgg_rev_P	ggaacaaatccgtctagtaaactgagg
mouse72	sAg_bs_r9_12_atgt_fw_E	aattcctcagtttactagatgtatttgttcctgca
mouse73	sAg_bs_r9_12_atgt_rev_P	ggaacaaatacatctagtaaactgagg
mouse74	sAg_bs_m10cg_fw_E	aattcctcagtttactagtggcatttgttcctgca
mouse75	sAg_bs_m10cg_rev_P	ggaacaaatgccactagtaaactgagg
mouse76	sAg_bs_m10ca_fw_E	aattcctcagtttactagtgacatttgttcctgca
mouse77	sAg_bs_m10ca_rev_P	ggaacaaatgtcactagtaaactgagg
mouse78	sAg_bs_m11gc_fw_E	aattcctcagtttactagtcccatttgttcctgca
mouse79	sAg_bs_m11gc_rev_P	ggaacaaatgggactagtaaactgagg
mouse80	sAg_bs_m11ga_fw_E	aattcctcagtttactagtaccatttgttcctgca
mouse81	sAg_bs_m11ga_rev_P	ggaacaaatggtactagtaaactgagg
mouse82	sAg_bs_onlyseed_fw_E	aattcctcagtaatgatcacggatttgttcctgca
mouse83	sAg_bs_onlyseed_rev_P	ggaacaaatccgtgatcattactgagg
mouse84	sAg_bs_r16-18_fw_E	aattcctcagttatgtagtgccatttgttcctgca
mouse85	sAg_bs_r16-18_rev_P	ggaacaaatggcactacataactgagg

Modeling Training and Validation Binding Site Set-miRsAg

ID	Name	Sequence (5' to 3')
miRsAg001	fw_model_sAg_Bs_1	ccgggataatttgttcatttgttctctagagc
miRsAg002	fw_model_sAg_Bs_2	ccgggataatttgttcatttgttcatttgttctctagagc
miRsAg003	fw_model_sAg_Bs_3	ccgggagtttactagtgccatttgttcaaatatagcctctagagc
miRsAg004	fw_model_sAg_Bs_4	ccgggagtttactagtgcaatttgttaaaatttagcctctagagc
miRsAg005	fw_model_sAg_Bs_5	ccgggagtttactagtgaaatttgttaaaatttagcctctagagc
miRsAg006	fw_model_sAg_Bs_6	ccgggagtttactagtaaaatttgttaaaatttagcctctagagc
miRsAg007	fw_model_sAg_Bs_7	ccgggagtttactagaaaaatttgttaaaatttagcctctagagc
miRsAg008	fw_model_sAg_Bs_8	ccgggctgggcaattataaatttgttaaaatttagcctctagagc
miRsAg009	fw_model_sAg_Bs_9	ccgggctgggcagccgcaaatttgttaaaggcccgcctctagagc
miRsAg010	fw_model_sAg_Bs_10	ccgggctgggcagctataattttgttaaaatttagcctctagagc
miRsAg011	fw_model_sAg_Bs_11	ccgggagtttacgccgtaaatttgttgaaggcccgcctctagagc
miRsAg012	fw_model_sAg_Bs_12	ccgggctgggcaattataaatttgttgaaatttagcctctagagc
miRsAg013	fw_model_sAg_Bs_13	ccgggtccttactagtgcaatttgttaaaggcccgcctctagagc
miRsAg014	fw_model_sAg_Bs_14	ccgggctgaatatagtgaaatttgttaaaatttagcctctagagc
miRsAg015	fw_model_sAg_Bs_15	ccgggagtttactacctaattttgttaaaatccggcctctagagc
miRsAg016	fw_model_sAg_Bs_16	ccgggctgggcctagtggattttgttaaaggcccgcctctagagc
miRsAg017	fw_model_sAg_Bs_17	ccgggagtttacattgcaaatttgttgaaggcccgcctctagagc
miRsAg018	fw_model_sAg_Bs_18	ccgggctgggactagtgcaatttgttgaaggcccgcctctagagc
miRsAg019	fw_model_sAg_Bs_20	ccgggagtttactagaaaattttgttaaaatttagcctctagagc
miRsAg020	fw_model_sAg_Bs_21	ccgggagtttactagaaaaatttgttgaaatttagcctctagagc
miRsAg021	fw_model_sAg_Bs_22	ccgggagtttactccgcaaccgctaggaaagcccgcctctagagc
miRsAg022	fw_model_sAg_Bs_23	ccgggctgggcatagataattttgttaaaatttagcctctagagc
miRsAg023	fw_model_sAg_Bs_24	ccgggctggtactagctaattttgttaaaatccggcctctagagc
miRsAg024	fw_model_sAg_Bs_25	ccgggagtttactagccgattttgttaaaggcccgcctctagagc
miRsAg025	fw_model_sAg_Bs_26	ccgggctgggcctagaaaaatttgttgaaatccggcctctagagc
miRsAg026	fw_model_sAg_Bs_27	ccgggcttttactagaaaaatttgttgaatttagcctctagagc
miRsAg027	fw_model_sAg_Bs_28	ccgggctggtactaggcaaatttgttgaaggcccgcctctagagc
miRsAg028	fw_model_sAg_Bs_29	ccggggctttactagaaaaatttgttaaaatttagcctctagagc
miRsAg029	fw_model_sAg_Bs_30	ccgggagtttactttaaaaatttgttaaaatttagcctctagagc
miRsAg030	rev_model_sAg_Bs_1	tcgagctctagagaacaaatgaacaaattatc
miRsAg031	rev_model_sAg_Bs_2	tcgagctctagagaacaaatgaacaaatgaacaaattatc
miRsAg032	rev_model_sAg_Bs_3	tcgagctctagaggctatatttgaacaaatggcactagtaaactc
miRsAg033	rev_model_sAg_Bs_4	tcgagctctagaggctaaattttaacaaattgcactagtaaactc
miRsAg034	rev_model_sAg_Bs_5	tcgagctctagaggctaaattttaacaaatttcactagtaaactc
miRsAg035	rev_model_sAg_Bs_6	tcgagctctagaggctaaattttaacaaattttactagtaaactc
miRsAg036	rev_model_sAg_Bs_7	tcgagctctagaggctaaattttaacaaatttttctagtaaactc
miRsAg037	rev_model_sAg_Bs_8	tcgagctctagaggctaaattttaacaaatttataattgcccagc
miRsAg038	rev_model_sAg_Bs_9	tcgagctctagaggcgggcctttaacaaatttgcggctgcccagc
miRsAg039	rev_model_sAg_Bs_10	tcgagctctagaggctaaattttaacaaaattatagctgcccagc
miRsAg040	rev_model_sAg_Bs_11	tcgagctctagaggcgggccttcaacaaatttacggcgtaaactc
miRsAg041	rev_model_sAg_Bs_12	tcgagctctagaggctaaatttcaacaaatttataattgcccagc
miRsAg042	rev_model_sAg_Bs_13	tcgagctctagaggcgggcctttaacaaattgcactagtaaggac
miRsAg043	rev_model_sAg_Bs_14	tcgagctctagaggctaaattttaacaaatttcactatattcagc
miRsAg044	rev_model_sAg_Bs_15	tcgagctctagaggccggattttaacaaaattaggtagtaaactc
miRsAg045	rev_model_sAg_Bs_16	tcgagctctagaggcgggcctttaacaaaatccactaggcccagc
miRsAg046	rev_model_sAg_Bs_17	tcgagctctagaggcgggccttcaacaaatttgcaatgtaaactc
miRsAg047	rev_model_sAg_Bs_18	tcgagctctagaggcgggccttcaacaaattgcactagtcccagc
miRsAg048	rev_model_sAg_Bs_20	tcgagctctagaggctaaattttaacaaaattttctagtaaactc
miRsAg049	rev_model_sAg_Bs_21	tcgagctctagaggctaaatttcaacaaatttttctagtaaactc
miRsAg050	rev_model_sAg_Bs_22	tcgagctctagaggcgggctttcctagcggttgcggagtaaactc
miRsAg051	rev_model_sAg_Bs_23	tcgagctctagaggctaaattttaacaaaattatctatgcccagc
miRsAg052	rev_model_sAg_Bs_24	tcgagctctagaggccggattttaacaaaattagctagtaccagc
miRsAg053	rev_model_sAg_Bs_25	tcgagctctagaggcgggcctttaacaaaatcggctagtaaactc
miRsAg054	rev_model_sAg_Bs_26	tcgagctctagaggccggatttcaacaaatttttctaggcccagc
miRsAg055	rev_model_sAg_Bs_27	tcgagctctagaggctaaattcaacaaatttttctagtaaaagc
miRsAg056	rev_model_sAg_Bs_28	tcgagctctagaggcgggccttcaacaaatttgcctagtaccagc
miRsAg057	rev_model_sAg_Bs_29	tcgagctctagaggctaaattttaacaaatttttctagtaaagcc
miRsAg058	rev_model_sAg_Bs_30	tcgagctctagaggctaaattttaacaaatttttaaagtaaactc
miRsAg059	Luc2_BS_seq_screen	acgacgatgccggcgagctg

@@ Line 580: / Line 580: @@
-==maus oligos==
+==mouse oligos==
 {| border="1" class="wikitable zebra sortable" cellpadding="6" style="border:solid 1px #AAAAAA; border-collapse:collapse; background-color:#F9F9F9; font-size:95%; empty-cells:show;"
@@ Line 755: / Line 755: @@
 |align="right"| mouse85 || sAg_bs_r16-18_rev_P || ggaacaaatggcactacataactgagg
 |}
 ==Modeling Training and Validation Binding Site Set-miRsAg==