Team:SDU-Denmark/labnotes

(Difference between revisions)

Revision as of 13:00, 9 July 2010

Group: Flagella

Experiment: Extraction of psb3k3 plasmids with incerted RFP from E. coli MG1655 (log phase)

Methods: MiniPrep, NanoDrop and gel electrophoresis

Notes: We used 9 ml ON culture, loaded 2ul sample and 8ul agarose loading dye on a 1,5% gel,used a DNA ladder mix (100-10000 nucleotides) as marker

Results: Nanodrop: Tube 1: 30.7 mg/ul Tube 2: 27.4 mg/ul

Gel electrophoresis: No result

--Louch07 12:55, 9 July 2010 (UTC)

@@ Line 12: / Line 12: @@
 ''Methods:'' MiniPrep, NanoDrop and gel electrophoresis
-''Notes:'' We used 9 ml ON culture
+''Notes:'' We used 9 ml ON culture, loaded 2ul sample and 8ul agarose loading dye on a 1,5% gel,used a DNA ladder mix (100-10000 nucleotides) as marker
-''Results:''
+''Results:'' Nanodrop: Tube 1: 30.7 mg/ul Tube 2: 27.4 mg/ul
-Nanodrop:
+Gel electrophoresis: No result
-Tube 1: 30.7 mg/ul
-Tube 2: 27.4 mg/ul
-Gel electrophoresis:
-No result
 --[[User:Louch07|Louch07]] 12:55, 9 July 2010 (UTC)