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Team:Chiba/Plasmid1
From 2010.igem.org
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<font size="5">PT7/cI hybrid promoter</font> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K396000">BBa_K396000</a><br> | <font size="5">PT7/cI hybrid promoter</font> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K396000">BBa_K396000</a><br> | ||
low unregulated, repressed by cI, activated by T7, repression is stronger than activation.<br><br> | low unregulated, repressed by cI, activated by T7, repression is stronger than activation.<br><br> | ||
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<table border="0" cellpadding="30" cellspacing="0"> | <table border="0" cellpadding="30" cellspacing="0"> | ||
<tr> | <tr> | ||
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<hr width="500" size="1" align="left"><br> | <hr width="500" size="1" align="left"><br> | ||
<font size="2" face=verdana> | <font size="2" face=verdana> | ||
| - | + | T7/CI-OR1 hybrid promoter was designed to be activated by T7 RNA Polymerase and repressed by lambda CI protein. In sequence design, lambda CI operator site 1 (OR1, Part of BBa_R1051) was directly attached to the downstream of T7 promoter sequence (BBa_I719005). This characterization was implemented to validate the promoter function. In results, the activation and repression was actually observed. | |
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| + | <br> | ||
</font></td> | </font></td> | ||
| + | </tr> | ||
| + | </table> | ||
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| + | <table border="0" cellpadding="30" cellspacing="0"> | ||
| + | <tr> | ||
| + | <td width="800px"><font size="5" face=verdana>Experiments</font><br> | ||
| + | <hr width="500" size="1" align="left"><br> | ||
| + | <font size="3">Materials and Methods</font><br><br> | ||
| + | |||
</tr> | </tr> | ||
</table> | </table> | ||
</html> | </html> | ||
| - | [[Image: | + | [[Image:T7-CI-1.png|frame|center|Fig. A materials and methods]] |
<html> | <html> | ||
| + | <table border="0" cellpadding="30" cellspacing="0"> | ||
| + | <tr><td> | ||
| + | </tr></td> | ||
| + | <br><br> | ||
| + | <tr> | ||
| + | <td width="800px"><font size="5" face=verdana>Results</font><br> | ||
| + | <hr width="500" size="1" align="left"><br> | ||
| + | <font size="2" face=verdana> | ||
| + | Under the situation of CI non-expressed , T7 RNAP causes GFP expression by activating T7/CI hybrid | ||
| + | promoter (Fig.1). On the other hand, under the situation of CI expressed, T7 RNAP does not cause GFP | ||
| + | expression (Fig.2). The reason of this is considered as following. Under the situation of CI expressed, | ||
| + | even though T7 RNAP tries to activate T7/CI promoter, the promoter Is repressed by CI because the | ||
| + | ability of CI repression is superior to the one of T7 activation for this promoter. So it is concluded that | ||
| + | T7/CI hybrid promoter is to be activated by T7 RNA Polymerase and repressed by lambda CI protein, | ||
| + | following the rules of CI repression > T7 activation. | ||
| + | </font></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | </html> | ||
| + | [[Image:CI-2.png|frame|center|Fig. B Results]] | ||
| + | [[Image:CI-3.jpg|frame|center|Fig. C Supplementary Data]] | ||
| + | <html> | ||
| + | <table border="0" cellpadding="30" cellspacing="0"> | ||
| + | <tr> | ||
| + | <td width="800px"><font size="5" face=verdana>Reference</font><br> | ||
| + | <hr width="500" size="1" align="left"><br> | ||
| + | <font size="3"> | ||
| + | <br>1) | ||
| + | <br>2) | ||
| + | </td> | ||
| + | </td> | ||
| + | </table> | ||
</html> | </html> | ||
Revision as of 12:40, 27 October 2010
PT7/cI hybrid promoter BBa_K396000
low unregulated, repressed by cI, activated by T7, repression is stronger than activation.
| T7/CI-OR1 hybrid promoter T7/CI-OR1 hybrid promoter was designed to be activated by T7 RNA Polymerase and repressed by lambda CI protein. In sequence design, lambda CI operator site 1 (OR1, Part of BBa_R1051) was directly attached to the downstream of T7 promoter sequence (BBa_I719005). This characterization was implemented to validate the promoter function. In results, the activation and repression was actually observed. |
| Experiments Materials and Methods |
Fig. A materials and methods
| Results Under the situation of CI non-expressed , T7 RNAP causes GFP expression by activating T7/CI hybrid promoter (Fig.1). On the other hand, under the situation of CI expressed, T7 RNAP does not cause GFP expression (Fig.2). The reason of this is considered as following. Under the situation of CI expressed, even though T7 RNAP tries to activate T7/CI promoter, the promoter Is repressed by CI because the ability of CI repression is superior to the one of T7 activation for this promoter. So it is concluded that T7/CI hybrid promoter is to be activated by T7 RNA Polymerase and repressed by lambda CI protein, following the rules of CI repression > T7 activation. |
Fig. B Results
Fig. C Supplementary Data
| Reference 1) 2) |
