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Team:Chiba/Plasmid1
From 2010.igem.org
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<font size="5">PT7/cI hybrid promoter</font> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K396000">BBa_K396000</a><br> | <font size="5">PT7/cI hybrid promoter</font> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K396000">BBa_K396000</a><br> | ||
low unregulated, repressed by cI, activated by T7, repression is stronger than activation.<br><br> | low unregulated, repressed by cI, activated by T7, repression is stronger than activation.<br><br> | ||
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<table border="0" cellpadding="30" cellspacing="0"> | <table border="0" cellpadding="30" cellspacing="0"> | ||
<tr> | <tr> | ||
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【Abstract】 T7/CI-OR1 hybrid promoter was designed to be activated by T7 RNA Polymerase and repressed by lambda CI protein. In sequence design, lambda CI operator site 1 (OR1, Part of BBa_R1051) was directly attached to the downstream of T7 promoter sequence (BBa_I719005). This characterization was implemented to validate the promoter function. In results, the activation and repression was actually observed. | 【Abstract】 T7/CI-OR1 hybrid promoter was designed to be activated by T7 RNA Polymerase and repressed by lambda CI protein. In sequence design, lambda CI operator site 1 (OR1, Part of BBa_R1051) was directly attached to the downstream of T7 promoter sequence (BBa_I719005). This characterization was implemented to validate the promoter function. In results, the activation and repression was actually observed. | ||
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Revision as of 11:48, 27 October 2010
PT7/cI hybrid promoter BBa_K396000
low unregulated, repressed by cI, activated by T7, repression is stronger than activation.
| T7/CI-OR1 hybrid promoter 【Abstract】 T7/CI-OR1 hybrid promoter was designed to be activated by T7 RNA Polymerase and repressed by lambda CI protein. In sequence design, lambda CI operator site 1 (OR1, Part of BBa_R1051) was directly attached to the downstream of T7 promoter sequence (BBa_I719005). This characterization was implemented to validate the promoter function. In results, the activation and repression was actually observed. |
