Team:Chiba/Plasmid1

From 2010.igem.org

(Difference between revisions)
Line 183: Line 183:
<br><br><br>
<br><br><br>
<font size="5">PT7/cI hybrid promoter</font>&nbsp;&nbsp;&nbsp;<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K396000">BBa_K396000</a><br>
<font size="5">PT7/cI hybrid promoter</font>&nbsp;&nbsp;&nbsp;<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K396000">BBa_K396000</a><br>
-
&nbsp;&nbsp;&nbsp; low unregulated, repressed by cI, activated by T7, repression is stronger than activation.
+
&nbsp;&nbsp;&nbsp; low unregulated, repressed by cI, activated by T7, repression is stronger than activation.<br><br>
 +
<html>
 +
<table border="0" cellpadding="30" cellspacing="0">
 +
<tr>
 +
  <td width="800px"><font size="5" face=verdana>T7/CI-OR1 hybrid promoter</font><br>
 +
  <hr width="500" size="1" align="left"><br>
 +
  <font size="2" face=verdana>
 +
【Abstract】 T7/CI-OR1 hybrid promoter was designed to be activated by T7 RNA Polymerase and repressed by lambda CI protein. In sequence design,  lambda CI operator site 1 (OR1, Part of BBa_R1051) was directly attached to the downstream of T7 promoter sequence (BBa_I719005). This characterization was implemented to validate the promoter function. In results, the activation and repression was actually observed.
 +
 
 +
</font></td> 
 +
 
</html>
</html>

Revision as of 11:37, 27 October 2010




 




PT7/cI hybrid promoter   BBa_K396000
    low unregulated, repressed by cI, activated by T7, repression is stronger than activation.

Retrieved from "http://2010.igem.org/Team:Chiba/Plasmid1"
T7/CI-OR1 hybrid promoter

【Abstract】 T7/CI-OR1 hybrid promoter was designed to be activated by T7 RNA Polymerase and repressed by lambda CI protein. In sequence design, lambda CI operator site 1 (OR1, Part of BBa_R1051) was directly attached to the downstream of T7 promoter sequence (BBa_I719005). This characterization was implemented to validate the promoter function. In results, the activation and repression was actually observed.