Team:Heidelberg/Modeling/miBSdesigner

From 2010.igem.org

(Difference between revisions)
Line 1: Line 1:
-
{{:Team:Heidelberg/Double}}
+
{{:Team:Heidelberg/Template}}
{{:Team:Heidelberg/Pagetop|mibsdesigner}}
{{:Team:Heidelberg/Pagetop|mibsdesigner}}
<!--<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">-->
<!--<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">-->
Line 8: Line 8:
<style type="text/css">
<style type="text/css">
.wrapper {width: 260px; text-align:center;}
.wrapper {width: 260px; text-align:center;}
-
.wrapper2 {margin:auto; height: 750px; text-align:left; }
+
.wrapper2 {margin:auto; height: 600px; text-align:left; }
.title {padding-left:0px; line-height:2; font-weight:bold; font-size:17px;}
.title {padding-left:0px; line-height:2; font-weight:bold; font-size:17px;}
.option {padding-left:10px; white-space:pre-wrap;}
.option {padding-left:10px; white-space:pre-wrap;}
Line 27: Line 27:
<div class="t1">
<div class="t1">
Construction of microRNA binding sites </div><br>
Construction of microRNA binding sites </div><br>
-
<div style="width:350px; text-align:right;"><span class="help"><a href="#">?</a><span></div>
+
<div style="width:350px; text-align:right;"><span class="help"><a href="https://2010.igem.org/Team:Heidelberg/Modeling/trainingset#Input">?</a><span></div>
<div class="largetext" style="width:450px;">
<div class="largetext" style="width:450px;">
Sequence name<br>
Sequence name<br>
Line 38: Line 38:
<div class="largetext" style="width:400px;">
<div class="largetext" style="width:400px;">
<input name="quality" value="perfect" onchange="chMd()" checked="checked" type="radio">
<input name="quality" value="perfect" onchange="chMd()" checked="checked" type="radio">
-
Perfect Binding Site <span class="help" style="float:right;"><a href="#">?</a></span><br>
+
Perfect Binding Site <br>
<input name="quality" value="bulge" onchange="chMd()" type="radio">
<input name="quality" value="bulge" onchange="chMd()" type="radio">
Imperfect Binding Site with 4 nt bulge (9-12)<br>
Imperfect Binding Site with 4 nt bulge (9-12)<br>
Line 49: Line 49:
<td width=290px>
<td width=290px>
<div class="title">
<div class="title">
-
Seed sequence (1-8) &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span class="help"><a href="#" title="Seed sequence help">?</a></span>
+
Seed sequence (1-8) &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span class="help"><a href="https://2010.igem.org/Team:Heidelberg/Modeling/trainingset#Seed_Types" title="Seed sequence help">?</a></span>
</div>
</div>
<div class="option"><SELECT name="seed" onchange="chMd1()" disabled="disabled">
<div class="option"><SELECT name="seed" onchange="chMd1()" disabled="disabled">
Line 66: Line 66:
<td>
<td>
<div class="title">
<div class="title">
-
Supplementary region &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span class="help"><a href="#">?</a></span></div>
+
Supplementary region &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span class="help"><a href="https://2010.igem.org/Team:Heidelberg/Modeling/trainingset#Supplementary_Region">?</a></span></div>
<div class="option"><SELECT name="comp" onchange="chMd2()" disabled="disabled">
<div class="option"><SELECT name="comp" onchange="chMd2()" disabled="disabled">
<OPTION value="0">
<OPTION value="0">
Line 87: Line 87:
<td>
<td>
<div class="title">
<div class="title">
-
Modify AU content &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span class="help"><a href="#">?</a></span></div>
+
Modify AU content &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span class="help"><a href="https://2010.igem.org/Team:Heidelberg/Modeling/trainingset#AU_Content">?</a></span></div>
<table style="line-height:2; font-size:12px;">
<table style="line-height:2; font-size:12px;">
<tr>
<tr>
Line 104: Line 104:
</td>
</td>
<td>
<td>
-
<div class="title">Sticky ends for integration into plasmid &nbsp; <span class="help"><a href="#">?</a></span></div></div>
+
<div class="title">Sticky ends for integration into plasmid &nbsp; <span class="help"><a href="https://2010.igem.org/Team:Heidelberg/Modeling/trainingset#Sticky_Ends">?</a></span></div></div>
<div class="option"><SELECT id="stickyends" name="stickyends" onchange="chMd3()">
<div class="option"><SELECT id="stickyends" name="stickyends" onchange="chMd3()">
<OPTION SELECTED value="none">None
<OPTION SELECTED value="none">None

Revision as of 10:57, 27 October 2010

BS designer for miRNA
Construction of microRNA binding sites

Sequence name

microRNA Sequence ( 5' -> 3')

Spacer (inert sequence)


Perfect Binding Site
Imperfect Binding Site with 4 nt bulge (9-12)
Personalized Binding Site

Seed sequence (1-8)              ?
Customized MISMATCH position
Supplementary region        ?
Customized MATCH positions
Modify AU content                ?
A in position -1
A in position 1
A in position 9
 A in position 0
A in position 8
A in position 10
Sticky ends for integration into plasmid   ?
Customized:
5'  
3'  



Name of primer 1


Sequence of primer 1


Name of primer 2


Sequence of primer 2

Retrieved from "http://2010.igem.org/Team:Heidelberg/Modeling/miBSdesigner"