Revision as of 09:39, 27 October 2010

TEAM ALBERTA

Transforming Cells

Project Timeline: Click on an image to see more information

Transformation typically requires expensive equipment and storing competent cells is difficult for many high schools who do not have -80^oC freezers. We tried a few methods of transforming cells and making them competent to attempt to solve this problem in our kit.

TSS Transformation Protocol

The TSS transformation was tested. The procedure is listed here.

One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution

http://www.pnas.org/content/86/7/2172.abstract

Acquisition of reagents- June 3, 2010
Preparation of buffer - June 7, 2010
Cell preparation - June 7, 2010
Competency - June 10, 2010

Rubidium chloride competent cells and lyophilization

This method was adapted from a method used by Justin Fedor.

Preparation of transformation buffer - July 6, 2010
Preparation of cells - July 10, 2010
Preparing competent cells - July 11, 2010
Lyophilization - July 13, 2010
Transformation of FD cells - July 14, 2010
Preparation of cells 2 - July 30, 2010
Lyophilization - August 2, 2010
Transformation - August 5, 2010

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 Transformation typically requires expensive equipment and storing competent cells is difficult for many high schools who do not have -80<sup>o</sup>C freezers.
 We tried a few methods of transforming cells and making them competent to attempt to solve this problem in our kit.

Team:Alberta/Notebook/TransformingCells

From 2010.igem.org