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Team:Alberta/Notebook/TransformingCells
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Transformation typically requires expensive equipment and storing competent cells is difficult for many high schools who do not have -80<sup>o</sup>C freezers. | Transformation typically requires expensive equipment and storing competent cells is difficult for many high schools who do not have -80<sup>o</sup>C freezers. | ||
Revision as of 08:56, 27 October 2010
Transforming Cells
Transformation typically requires expensive equipment and storing competent cells is difficult for many high schools who do not have -80oC freezers. We tried a few methods of transforming cells and making them competent to attempt to solve this problem in our kit.
TSS Transformation Protocol
The TSS transformation was tested. The procedure is listed here.
One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution
http://www.pnas.org/content/86/7/2172.abstract
- Acquisition of reagents- June 3, 2010
- Preparation of buffer - June 7, 2010
- Cell preparation - June 7, 2010
- Competency - June 10, 2010
Rubidium chloride competent cells and lyophilization
This method was adapted from a method used by Justin Fedor.
- Preparation of transformation buffer - July 6, 2010
- Preparation of cells - July 10, 2010
- Preparing competent cells - July 11, 2010
- Lyophilization - July 13, 2010
- Transformation of FD cells - July 14, 2010
- Preparation of cells 2 - July 30, 2010
- Lyophilization - August 2, 2010
- Transformation - August 5, 2010
