Team:Chiba/lux promoter

From 2010.igem.org

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[[Image:lux inverter function.png|frame|center|Fig. 2 assay process]]
[[Image:lux inverter function.png|frame|center|Fig. 2 assay process]]
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<br>1) Egland.K.A, and Greenberg.E.P, Conversion of the ''Vibrio Fischeri'' Transcriptional Activator,LuxR, to a Repressor, ''J. Bacteriol.'', '''182''', P.805-811 (2000)
<br>1) Egland.K.A, and Greenberg.E.P, Conversion of the ''Vibrio Fischeri'' Transcriptional Activator,LuxR, to a Repressor, ''J. Bacteriol.'', '''182''', P.805-811 (2000)
<br>2) Cox.R.S.3rd, Surette.M.G, Elowitz.M.B, Programming gene expression with combinatorial promoters, ''Mol Syst Biol'', '''3''' ,145 (2007)
<br>2) Cox.R.S.3rd, Surette.M.G, Elowitz.M.B, Programming gene expression with combinatorial promoters, ''Mol Syst Biol'', '''3''' ,145 (2007)

Revision as of 08:46, 27 October 2010




 

Lux promoter inverter


We've charaterized Plux inverter.
There is promoter (HSL-luxR repressor), biobrick number R0061.
We've made Plux inv-GFP and characterized about it.

Experiments


1.Preparing Plux inv-GFP

   cloning process is shown in Fig. 1

Fig. 1 cloning process

Results


Fig. 2 assay process


Reference



1) Egland.K.A, and Greenberg.E.P, Conversion of the Vibrio Fischeri Transcriptional Activator,LuxR, to a Repressor, J. Bacteriol., 182, P.805-811 (2000)
2) Cox.R.S.3rd, Surette.M.G, Elowitz.M.B, Programming gene expression with combinatorial promoters, Mol Syst Biol, 3 ,145 (2007)