Team:MIT projectContext

Bacteria

• Overview
• Toggle Construction
• Characterization

Phage

• Introduction
• Background
• Design
• Construction
• Results
• Context

Mammalian

• Overview
• New Mammalian Standard
• Circuit Design
• Mechanosensation
• Bone Formation
• Synthetic Switch

Phage

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WORK FROM OTHER IGEM TEAMS
The following teams have used some component of our phage system previously. No other teams have used our method of polyphage with incorporated leucine zippers for polymerization (nor is it present in the literature).

• In 2006 the McGill team attempted to use leucine zippers fused to split YFP to display on cells and cause the cells to adhere via the split YFP. See: http://parts.mit.edu/wiki/index.php/McGill_University_2006



• The 2009 Freiburg Bioware team used Fos/Jun for a 'programmable enzyme' using Fok-fused Fos/Jun as factor in DNA cleavage. See: https://2009.igem.org/Team:Freiburg_bioware/Project/FOS



• Paris's team in 2009 attempted to use Jun/Fos as a snare; Jun on signal vesicle, Fos on membrane of receiving cell. See: https://2009.igem.org/Team:Paris#top



• This year, Duke's team is using Jun- and Fos-based synthetic leucine zippers for genetic regulation. See: https://2010.igem.org/Team:Duke/Project#Leucine_Zippers

RELEVANT LITERATURE

• Rackonjac and Model. "Roles of pIII is Filamentous Phage Assembly". J Mol Bio. 1998.
• Barbas III, et al. "Phage Display: A Laboratory Manual". CSH Press. 2001.
• Wang, et al. "Adapter-directed display: a modular design for shuttling display on phage surfaces". J Mol Bio. 2010.
• Smith and Petrenko. "Phage Display". Chemical Review. 1997.
• Sweeney, et al. "Assembly of Multimeric Phage Nanostructures Through Leucine Zipper Interactions". Biotechnology and Bioengineering. 2006.
• Roth, et al. "A Minimized M13 Coat Protein Defines the Requirements for Assembly into the Bacteriophage Particle". J Mol Bio. 2002.
• Weiss and Sidhu. "Design and Evolution of Artificial M13 Coat Proteins". J Mol Bio. 2000.

project context

Bacterial WORK FROM OTHER IGEM TEAMS In 2005, the UT Austin team worked on a light-dependent bacterial printer. RELEVANT LITERATURE Kobayashi, Collins, et al. "Programmable cells: Interfacing natural and engineered gene networks". PNAS. 2004. Crowl, et al. "Repressor cleavage as a prophage induction mechanism: Hypersensitivity of a mutant λ cI protein to RecA-mediated proteolysis". J Mol Bio. 1981. Gimble, Sauer. "Mutations in Bacteriophage X Repressor That Prevent RecA-Mediated Cleavage". Journal of Bacteriology. 1985.