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Team:Queens-Canada/15 July 2010
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(Difference between revisions)
(New page: <h2>July 15, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Liquid Culture</h3> Hao '''BBa_I13522''', '''pSip-'''::1A3 <h3>[[Team:Queens-Canad...) |
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<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3> | <h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3> | ||
Yuli and Thai | Yuli and Thai | ||
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'''pSB1A3'''::'''pSip-1''' | '''pSB1A3'''::'''pSip-1''' | ||
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<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3> | <h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3> | ||
Yuli | Yuli | ||
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'''pSB1A3'''::'''pSip-1''' | '''pSB1A3'''::'''pSip-1''' | ||
Latest revision as of 05:50, 27 October 2010
July 15, 2010
Liquid Culture
Hao
BBa_I13522, pSip-::1A3
Diagnostic PCR
Thai and Hao
pStr-1
Re-run blends A, D, H, K, L, and M from July 14th using an extension time of 15 s, and an annealing temperature of 55 °C.
Gel Electrophoresis
Geoff and Thai
Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 100 V.
Heat Shock
Chris
pRF4
Plates stored in 37 °C room overnight.
Digestion
Yuli and Thai
pSB1A3::pSip-1
Cut with XbaI and PstI.
Gel Electrophoresis
Yuli
pSB1A3::pSip-1
Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 100 V.
