Team:Queens-Canada/15 July 2010

Liquid Culture

Hao

BBa_I13522, pSip-::1A3

Diagnostic PCR

Thai and Hao

pStr-1

Re-run blends A, D, H, K, L, and M from July 14th using an extension time of 15 s, and an annealing temperature of 55 °C.

Gel Electrophoresis

Geoff and Thai

Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 100 V.

Heat Shock

Chris

pRF4

Plates stored in 37 °C room overnight.

Digestion

Yuli and Thai

pSB1A3::pSip-1

Cut with XbaI and PstI.

Gel Electrophoresis

Yuli

pSB1A3::pSip-1

Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 100 V.