Team:Queens-Canada/17 June 2010
From 2010.igem.org
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Used 1.1 g agarose in 160 mL TBE. Gel run for 40 mins at 100 V. | Used 1.1 g agarose in 160 mL TBE. Gel run for 40 mins at 100 V. | ||
- | + | <h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3> | |
- | Digestion | + | |
Nelson | Nelson | ||
Revision as of 05:23, 27 October 2010
Contents |
June 17, 2010
Gel Electrophoresis
Kevin and Nelson
pSip-1::eGFP, pHsp-3::eGFP, pMec-7::eGFP, pUnc-47::eGFP, pRab-7::eGFP
Used 1.1 g agarose in 160 mL TBE. Gel run for 40 mins at 100 V.
Digestion
Nelson
BBa_J45200, BBa_J45120
Incubated at 37 °C for 20 mins. Products stored at -20 °C.
Gel Electrophoresis
Yuli PCR Purification products from June 16, 2010
Used 0.4 g agarose in in 60 mL TBE. Gel run for 40 mins at 100 V.