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From 2010.igem.org

Contents 1 June 17, 2010 1.1 Gel Electrophoresis 1.2 Digestion 1.3 Gel Electrophoresis

June 17, 2010

Gel Electrophoresis

Kevin and Nelson

pSip-1::eGFP, pHsp-3::eGFP, pMec-7::eGFP, pUnc-47::eGFP, pRab-7::eGFP

Used 1.1 g agarose in 160 mL TBE. Gel run for 40 mins at 100 V.

Digestion

Nelson

BBa_J45200, BBa_J45120

Incubated at 37 °C for 20 mins. Products stored at -20 °C.

Gel Electrophoresis

Yuli

PCR Purification products from June 16, 2010

Used 0.4 g agarose in in 60 mL TBE. Gel run for 40 mins at 100 V.