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==GENOMIKON- Kit Design== | ==GENOMIKON- Kit Design== | ||
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'''The Idea'''<p> | '''The Idea'''<p> | ||
GENOMIKON evolved from a team objective to bring synthetic biology to high school students. High schools have limitations that don't apply to university research labs and thus we had to over come many design challenges. </p> | GENOMIKON evolved from a team objective to bring synthetic biology to high school students. High schools have limitations that don't apply to university research labs and thus we had to over come many design challenges. </p> | ||
<p>To work GENOMIKON must be: | <p>To work GENOMIKON must be: | ||
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*Require very little expensive equipment (autoclaves, centrifuges, -70C freezers) | *Require very little expensive equipment (autoclaves, centrifuges, -70C freezers) | ||
*Fast | *Fast | ||
Revision as of 04:33, 27 October 2010
GENOMIKON- Kit Design
The IdeaGENOMIKON evolved from a team objective to bring synthetic biology to high school students. High schools have limitations that don't apply to university research labs and thus we had to over come many design challenges.
To work GENOMIKON must be:
- Require very little expensive equipment (autoclaves, centrifuges, -70C freezers)
- Fast
- Efficient
- Easily shipped
- Inexpensive
BioBytes 2.0 solved most of these problems.
BioBytes 2.0 is:
- Fast: Parts can be added to a growing construct in 7 minutes.
- Efficient: It has been modeled to be 95% efficient. We have also used BioBytes 2.0 to create plasmids that have been transformed successfully into E. coli.
- Equipment: It does not require centrifugation or any other expensive equipment inherently.
The other limitations were solved by using innovative protocols and products:
- Equipment: Expensive micropipetters are replaced with precision droppers, which can accurately dispense small volumes of solutions.
- Easily Shipped: We will utilize lyophilized competent cells so that cold temperatures are not necessary.
- Inexpensive: Based on our distribution analysis, the kit will be inexpensive to produce and can be sold at costs that are lower than currently used high school biology labs.
GENOMIKON was designed with safety in mind and as such it has many safety features built in.
- A common lab strain of E. coli DH5α is used. This strain has been used safely in labs around the world and was designed to be safe.
- Proper safety equipment is not required, but will be recommended to further decrease the risks of contamination.
- The kit will teach safety education to the students.
When we tested our kit with high school students from the Edmonton area, it had a warm reception. They had great things to say about the educational aspect and the scientific aspect of the kit. They did give us some useful feedback. An in-depth look at this component can be seen on real world trials.
