"
Team:Cambridge/Tools/microMeasure
From 2010.igem.org
(→Co-reporters) |
(→Real life isn't black and white) |
||
| Line 7: | Line 7: | ||
==Real life isn't black and white== | ==Real life isn't black and white== | ||
{{:Team:Cambridge/Templates/RightImage|image=Cambridge-eglo.jpg|caption=3D mock-up of a mass produced bioluminescent biosensor featuring the same technology as our [https://2010.igem.org/Team:Cambridge/Tools/Eglometer E.glometer]: a cuvette for loading sample, simple buttons for making readings, LCD screen for output.}} | {{:Team:Cambridge/Templates/RightImage|image=Cambridge-eglo.jpg|caption=3D mock-up of a mass produced bioluminescent biosensor featuring the same technology as our [https://2010.igem.org/Team:Cambridge/Tools/Eglometer E.glometer]: a cuvette for loading sample, simple buttons for making readings, LCD screen for output.}} | ||
| - | In every application of a biosensor it is useful to know the '''amount''' of the factor being measured. There are a numerous existing reporters. However to our knowledge there are none which are both affordable and <strong>quantitative</strong>. Some teams attempt to solve this problem by using a series of wells sensitive to increasing concentrations of the substance. But this requires a great deal of work to tune the sensors correctly. | + | In every application of a biosensor it is useful to know the '''amount''' of the factor being measured. There are a numerous existing reporters. However to our knowledge there are none which are both affordable and <strong>quantitative</strong>. Some teams attempt to solve this problem by using a series of wells sensitive to increasing concentrations of the substance. But this requires a great deal of work to tune the sensors correctly and can add to their cost. |
We have already shown that the amount of the light produced by a bacterial culture can be assayed affordably with our [https://2010.igem.org/Team:Cambridge/Tools/Eglometer E.glometer]. Such devices could be mass produced lowering production costs further and distributed with the bacterial biosensors. | We have already shown that the amount of the light produced by a bacterial culture can be assayed affordably with our [https://2010.igem.org/Team:Cambridge/Tools/Eglometer E.glometer]. Such devices could be mass produced lowering production costs further and distributed with the bacterial biosensors. | ||
<html><div style="clear:both"></div></html> | <html><div style="clear:both"></div></html> | ||
| + | |||
==Co-reporters== | ==Co-reporters== | ||
It is often useful to have one cell measuring reporting two different outputs. Light is an effective means of achieving this, since it has different wavelengths which can be easily separated by inexpensive filters. The various [https://2010.igem.org/Team:Cambridge/Bioluminescence/Colour coloured outputs] that we have created would be useful tools for achieving this. | It is often useful to have one cell measuring reporting two different outputs. Light is an effective means of achieving this, since it has different wavelengths which can be easily separated by inexpensive filters. The various [https://2010.igem.org/Team:Cambridge/Bioluminescence/Colour coloured outputs] that we have created would be useful tools for achieving this. | ||
Latest revision as of 04:23, 27 October 2010
Real life isn't black and white
In every application of a biosensor it is useful to know the amount of the factor being measured. There are a numerous existing reporters. However to our knowledge there are none which are both affordable and quantitative. Some teams attempt to solve this problem by using a series of wells sensitive to increasing concentrations of the substance. But this requires a great deal of work to tune the sensors correctly and can add to their cost.
We have already shown that the amount of the light produced by a bacterial culture can be assayed affordably with our E.glometer. Such devices could be mass produced lowering production costs further and distributed with the bacterial biosensors.
Co-reporters
It is often useful to have one cell measuring reporting two different outputs. Light is an effective means of achieving this, since it has different wavelengths which can be easily separated by inexpensive filters. The various coloured outputs that we have created would be useful tools for achieving this.
Applications of co-reporters include:
- Using one output as a control to check for biosensor viability, avoiding false negatives
- Measuring two or more quantities concurrently, e.g. a number of different toxins
