Team:Caltech/Parts

From 2010.igem.org

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== Intermediate Parts ==
== Intermediate Parts ==
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Revision as of 02:20, 27 October 2010


iGEM 2010



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Completed Bricks

Heat shock promoter: BBa_K338001

HSP-LacI: BBa_K338002

Plastic, part 1: BBa_K338003

Plastic, part 2: BBa_K338004

Intermediate Parts

Part Name Brick Number Resistance Subparts
phaB1.RBS (plastic intermediate) <partinfo>BBa_K332023</partinfo> Cm <partinfo>BBa_K156013</partinfo>, <partinfo>BBa_B0034</partinfo>
Heat-Shock Activated Promoter <partinfo>BBa_K338001</partinfo> Amp Original part
Heat-Shock or IPTG Induced Generator <partinfo>BBa_K338002</partinfo> Kan <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_B0034</partinfo>
pLacI.RBS.phaA.RBS (plastic intermediate) <partinfo>BBa_K338003</partinfo> Kan <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_K156012</partinfo>, <partinfo>BBa_B0034</partinfo>
phaB1.RBS.phaC1.TT (plastic intermediate) <partinfo>BBa_K338004</partinfo> Kan <partinfo>BBa_K156013</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_K156014</partinfo>, <partinfo>BBa_B0015</partinfo>
phaA.RBS (plastic intermediate) <partinfo>BBa_K338022</partinfo> Cm <partinfo>BBa_K156012</partinfo>, <partinfo>BBa_B0034</partinfo>
phaC1 with Double Terminator <partinfo>BBa_K338024</partinfo> Cm <partinfo>BBa_K156014</partinfo>, <partinfo>BBa_B0015</partinfo>
+OmpR, +(CinR-HSL) Double Promoter <partinfo>BBa_K338029</partinfo> Tet <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_R0077</partinfo>
GFP Reporter Device, Heat-Shock Induced <partinfo>BBa_K338042</partinfo> Kan <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
+Heat-Shock, +CinR-HSL Double Promoter <partinfo>BBa_K338046</partinfo> Kan <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_R0077</partinfo>
GFP Reporter Device, Heat-Shock or IPTG Induced <partinfo>BBa_K338062</partinfo> Cm <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
Heat-Shock Activated Generator <partinfo>BBa_K338081</partinfo> Kan <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_B0034</partinfo>
GFP Reporter Device, Cinr-HSL Induced Not Submitted Cm <partinfo>BBa_R0077</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
HSL with Double Terminator Not Submitted Cm <partinfo>BBa_C0076</partinfo>, <partinfo>BBa_B0015</partinfo>
Constitutive Generator Not Submitted Kan <partinfo>BBa_J23119</partinfo>, <partinfo>BBa_B0034</partinfo>
CinR with Double Terminator Not Submitted Cm <partinfo>BBa_C0077</partinfo>, <partinfo>BBa_B0015</partinfo>
+IPTG, +(CinR-HSL) Double Promoter Not Submitted Cm <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_R0077</partinfo>
OmpR Induced Generator Not Submitted Tet <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_B0034</partinfo>
HSL.TT.pLacI.p(CinR-HSL) (intermediate) Not Submitted Amp <partinfo>BBa_C0076</partinfo>, <partinfo>BBa_B0015</partinfo>, <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_R0077</partinfo>
HSL Device, CinR-HSL Induced Not Submitted Kan <partinfo>BBa_R0077</partinfo>, <partinfo>BBa_C0076</partinfo>, <partinfo>BBa_B0015</partinfo>
HSL Device Not Submitted Kan <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_C0076</partinfo>, <partinfo>BBa_B0015</partinfo>
Heat-Shock Activated Generator Not Submitted Kan <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_B0034</partinfo>
GFP Reporter Device, OmpR Induced Not Submitted Cm <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
GFP Reporter Device, Constitutive Not Submitted Kan <partinfo>BBa_J23119</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
K124014 Holin with Double Terminator Not Submitted Cm <partinfo>BBa_K124014</partinfo>, <partinfo>BBa_B0015</partinfo>
K124017 Lysis Cassette with Double Terminator Not Submitted Cm <partinfo>BBa_K124017</partinfo>, <partinfo>BBa_B0015</partinfo>
CinR Device, OmpR or CinR-HSL Induced Not Submitted Amp <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_R0077</partinfo>, <partinfo>BBa_C0077</partinfo>, <partinfo>BBa_B0015</partinfo>
CinR Device, OmpR Induced Not Submitted Amp <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_C0077</partinfo>, <partinfo>BBa_B0015</partinfo>


Apparatus

The red/infrared light apparatus

The red/infrared light emitting apparatus we built to test our heat shock promoter was modeled after the original [http://openwetware.org/wiki/LightCannon Light Cannon]. It features a theater lamp with a Roscolux #22 Deep Amber red/infrared light filter ([http://www.amazon.com/Rosco-Laboratories-22-ROSCO-Roscolux-22/dp/B000XRCGDE/ref=sr_1_1?ie=UTF8&s=electronics&qid=1287956720&sr=8-1 Amazon], [http://www.rosco.com/includes/filters/SED.asp?titleName=R22:%20Deep%20Amber&imageName=../../images/filters/roscolux/22.jpg Spectrum Data]).

We placed this light on the bench top above a rudimentary stage. By varying the height of the lamp above the stage, we could vary the temperature and light intensity at the stage. In the image, the stage temperature was measured to be 42°C.

Stage of the light apparatus; temperature at 42°C.
 
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