| Part Name
| Brick Number
| Resistance
| Subparts
|
| phaB1.RBS (plastic intermediate) | <partinfo>BBa_K332023</partinfo> | Cm | <partinfo>BBa_K156013</partinfo>, <partinfo>BBa_B0034</partinfo>
|
| Heat-Shock Activated Promoter | <partinfo>BBa_K338001</partinfo> | Amp | Original part
|
| Heat-Shock or IPTG Induced Generator | <partinfo>BBa_K338002</partinfo> | Kan | <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_B0034</partinfo>
|
| pLacI.RBS.phaA.RBS (plastic intermediate) | <partinfo>BBa_K338003</partinfo> | Kan | <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_K156012</partinfo>, <partinfo>BBa_B0034</partinfo>
|
| phaB1.RBS.phaC1.TT (plastic intermediate) | <partinfo>BBa_K338004</partinfo> | Kan | <partinfo>BBa_K156013</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_K156014</partinfo>, <partinfo>BBa_B0015</partinfo>
|
| phaA.RBS (plastic intermediate) | <partinfo>BBa_K338022</partinfo> | Cm | <partinfo>BBa_K156012</partinfo>, <partinfo>BBa_B0034</partinfo>
|
| phaC1 with Double Terminator | <partinfo>BBa_K338024</partinfo> | Cm | <partinfo>BBa_K156014</partinfo>, <partinfo>BBa_B0015</partinfo>
|
| +OmpR, +(CinR-HSL) Double Promoter | <partinfo>BBa_K338029</partinfo> | Tet | <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_R0077</partinfo>
|
| GFP Reporter Device, Heat-Shock Induced | <partinfo>BBa_K338042</partinfo> | Kan | <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
|
| +Heat-Shock, +CinR-HSL Double Promoter | <partinfo>BBa_K338046</partinfo> | Kan | <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_R0077</partinfo>
|
| GFP Reporter Device, Heat-Shock or IPTG Induced | <partinfo>BBa_K338062</partinfo> | Cm | <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
|
| Heat-Shock Activated Generator | <partinfo>BBa_K338081</partinfo> | Kan | <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_B0034</partinfo>
|
| GFP Reporter Device, Cinr-HSL Induced | Not Submitted | Cm | <partinfo>BBa_R0077</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
|
| HSL with Double Terminator | Not Submitted | Cm | <partinfo>BBa_C0076</partinfo>, <partinfo>BBa_B0015</partinfo>
|
| Constitutive Generator | Not Submitted | Kan | <partinfo>BBa_J23119</partinfo>, <partinfo>BBa_B0034</partinfo>
|
| CinR with Double Terminator | Not Submitted | Cm | <partinfo>BBa_C0077</partinfo>, <partinfo>BBa_B0015</partinfo>
|
| +IPTG, +(CinR-HSL) Double Promoter | Not Submitted | Cm | <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_R0077</partinfo>
|
| OmpR Induced Generator | Not Submitted | Tet | <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_B0034</partinfo>
|
| HSL.TT.pLacI.p(CinR-HSL) (intermediate) | Not Submitted | Amp | <partinfo>BBa_C0076</partinfo>, <partinfo>BBa_B0015</partinfo>, <partinfo>BBa_R0011</partinfo>, <partinfo>BBa_R0077</partinfo>
|
| HSL Device, CinR-HSL Induced | Not Submitted | Kan | <partinfo>BBa_R0077</partinfo>, <partinfo>BBa_C0076</partinfo>, <partinfo>BBa_B0015</partinfo>
|
| HSL Device | Not Submitted | Kan | <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_C0076</partinfo>, <partinfo>BBa_B0015</partinfo>
|
| Heat-Shock Activated Generator | Not Submitted | Kan | <partinfo>BBa_K338001</partinfo>, <partinfo>BBa_B0034</partinfo>
|
| GFP Reporter Device, OmpR Induced | Not Submitted | Cm | <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
|
| GFP Reporter Device, Constitutive | Not Submitted | Kan | <partinfo>BBa_J23119</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0040</partinfo>, <partinfo>BBa_B0015</partinfo>
|
| K124014 Holin with Double Terminator | Not Submitted | Cm | <partinfo>BBa_K124014</partinfo>, <partinfo>BBa_B0015</partinfo>
|
| K124017 Lysis Cassette with Double Terminator | Not Submitted | Cm | <partinfo>BBa_K124017</partinfo>, <partinfo>BBa_B0015</partinfo>
|
| CinR Device, OmpR or CinR-HSL Induced | Not Submitted | Amp | <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_R0077</partinfo>, <partinfo>BBa_C0077</partinfo>, <partinfo>BBa_B0015</partinfo>
|
| CinR Device, OmpR Induced | Not Submitted | Amp | <partinfo>BBa_R0082</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_C0077</partinfo>, <partinfo>BBa_B0015</partinfo>
|
The red/infrared light emitting apparatus we built to test our heat shock promoter was modeled after the original [http://openwetware.org/wiki/LightCannon Light Cannon]. It features a theater lamp with a Roscolux #22 Deep Amber red/infrared light filter ([http://www.amazon.com/Rosco-Laboratories-22-ROSCO-Roscolux-22/dp/B000XRCGDE/ref=sr_1_1?ie=UTF8&s=electronics&qid=1287956720&sr=8-1 Amazon], [http://www.rosco.com/includes/filters/SED.asp?titleName=R22:%20Deep%20Amber&imageName=../../images/filters/roscolux/22.jpg Spectrum Data]).
We placed this light on the bench top above a rudimentary stage. By varying the height of the lamp above the stage, we could vary the temperature and light intensity at the stage. In the image, the stage temperature was measured to be 42°C.
"
