Team:Yale/Our Project/Notebook

From 2010.igem.org

(Difference between revisions)
Line 88: Line 88:
<h4> Results & Conclusions </h4>
<h4> Results & Conclusions </h4>
-
<div align="left" style="margin: -5px" ><img src="https://static.igem.org/mediawiki/2010/3/37/Yale-dh5alpha.png" /><img src="https://static.igem.org/mediawiki/2010/7/75/Yale-le392.png" /></div>
+
<div align="center" style="margin: -5px" ><img src="https://static.igem.org/mediawiki/2010/3/37/Yale-dh5alpha.png" /></div>  
<ul>
<ul>
     <li> Both cells lines exhibit very similar copper tolerances, growing without noticeable effect at concentrations up to 500 uM, experiencing slowed growth at 1 mM, and no growth at 10 mM. </li>
     <li> Both cells lines exhibit very similar copper tolerances, growing without noticeable effect at concentrations up to 500 uM, experiencing slowed growth at 1 mM, and no growth at 10 mM. </li>

Revision as of 01:57, 27 October 2010

iGEM Yale

lab notebook: week 1 (6/7 - 6/13)

  • Monday 6/7--We successfully defend our project to our sponsors(yay!)
  • Wednesday 6/9 --First day in lab!--got set up & started cultures of two E. coli strains
  • See more/less
  • Thursday 6/10--inoculated liquid cultures in AM, made up different copper sulfate & LB solutions, then ran an assay measuring bacterial growth in the presence of copper, trying both strains at three different initial ODs and measuring for four hours
  • See more/less
  • Friday 6/11--Ran another growth assay with middling concentrations based on results of first, but bacteria failed to grow well--perhaps because allowed to overgrow prior to assay?
  • See more/less