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Team:Calgary/25 October 2010
From 2010.igem.org
Emily Hicks (Talk | contribs) |
H.dastidar (Talk | contribs) |
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Set up digests of cpxP, malE, malESSdel, I0500-I13507, K135000-I13507-I0500-B0034-malE31 and the psB1AC3 vector. This is to plasmid switch all of the remianing registry parts into the accepted shipping plasmid. After construction and Antarctic Phosphotase treatment, I ligated them and then transformed them into TOP10 Competant E. Coli cells and then innocultaed LB broth culture and left the cultures to grow overnight. Today I also preparted ovenright cultures for an arabinose induction in order to get some more characterizatuon data of the I0500 (arabinose inducible) promoter. I also entered our parts into the Registry of Standard Biological Prats in preparation for shipping tomorrow. | Set up digests of cpxP, malE, malESSdel, I0500-I13507, K135000-I13507-I0500-B0034-malE31 and the psB1AC3 vector. This is to plasmid switch all of the remianing registry parts into the accepted shipping plasmid. After construction and Antarctic Phosphotase treatment, I ligated them and then transformed them into TOP10 Competant E. Coli cells and then innocultaed LB broth culture and left the cultures to grow overnight. Today I also preparted ovenright cultures for an arabinose induction in order to get some more characterizatuon data of the I0500 (arabinose inducible) promoter. I also entered our parts into the Registry of Standard Biological Prats in preparation for shipping tomorrow. | ||
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| + | <u>Himika</u> | ||
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| + | Today I uploaded a whole bunch of wiki contents. I also subcultured and induced DegP cells containing MalE and MalE31 with 12 different arabinose concentrations. I also helped emily with a bunch of plasmid switches that failed before. | ||
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Latest revision as of 00:57, 27 October 2010
Monday October 25, 2010
Patrick
Assigned and obtained various paragraphs from other team members. Uploaded many on the Wiki.
Emily
Set up digests of cpxP, malE, malESSdel, I0500-I13507, K135000-I13507-I0500-B0034-malE31 and the psB1AC3 vector. This is to plasmid switch all of the remianing registry parts into the accepted shipping plasmid. After construction and Antarctic Phosphotase treatment, I ligated them and then transformed them into TOP10 Competant E. Coli cells and then innocultaed LB broth culture and left the cultures to grow overnight. Today I also preparted ovenright cultures for an arabinose induction in order to get some more characterizatuon data of the I0500 (arabinose inducible) promoter. I also entered our parts into the Registry of Standard Biological Prats in preparation for shipping tomorrow.
Himika
Today I uploaded a whole bunch of wiki contents. I also subcultured and induced DegP cells containing MalE and MalE31 with 12 different arabinose concentrations. I also helped emily with a bunch of plasmid switches that failed before.
