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Team:Calgary/2 July 2010
From 2010.igem.org
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| + | <u>Patrick</u> | ||
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| + | I attempted another restriction digest of ''cpxP'' in the hopes that I would get a better reading. There was, unfortunately, no separation of the bands. This suggests that all of the ''cpxP'' promoters have scarred in the plasmid, or that the plasmid had religated on itself. | ||
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| + | Thus, I will try and do a restriction digest using ''EcoRI'' and ''PstI'' sites in orer to see if the part is released at all. If the plasmid is cut with ''EcoRI'' and ''PstI'' but not ''XbaI'' and ''SpeI'', this definitely suggests that the part was facing in the wrong direction. | ||
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Friday July 2, 2010
Patrick
I attempted another restriction digest of cpxP in the hopes that I would get a better reading. There was, unfortunately, no separation of the bands. This suggests that all of the cpxP promoters have scarred in the plasmid, or that the plasmid had religated on itself.
Thus, I will try and do a restriction digest using EcoRI and PstI sites in orer to see if the part is released at all. If the plasmid is cut with EcoRI and PstI but not XbaI and SpeI, this definitely suggests that the part was facing in the wrong direction.
No notebook page exists for this date. Sorry!
Retrieved from "http://2010.igem.org/Team:Calgary/2_July_2010"
