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Team:UPO-Sevilla/Notebook/08 11
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<h2>Assembly Team</h2> | <h2>Assembly Team</h2> | ||
| - | <p> | + | <p>We had a lot of red and white colonies in every plate; except on: 12+19, 11+2+13+3 and 1+2+16+3 plates. There were no colonies on control plates. We made colony PCR with six colonies from each plate. Agarose gel analysis showed that running time was too long. We could not rule out anything, so we set up inocula of each candidate.</p> |
<p>We set up inocula of positive candidate from UPO 4, UPO 5 and UPO 11 plates. We made minipreps with the day before inocula and analytic digestions. 0.8% agarosa gel analysis confirm 2+18 and 2+4+2+6.</p> | <p>We set up inocula of positive candidate from UPO 4, UPO 5 and UPO 11 plates. We made minipreps with the day before inocula and analytic digestions. 0.8% agarosa gel analysis confirm 2+18 and 2+4+2+6.</p> | ||
Revision as of 00:01, 27 October 2010
August, 11th
Assembly Team
We had a lot of red and white colonies in every plate; except on: 12+19, 11+2+13+3 and 1+2+16+3 plates. There were no colonies on control plates. We made colony PCR with six colonies from each plate. Agarose gel analysis showed that running time was too long. We could not rule out anything, so we set up inocula of each candidate.
We set up inocula of positive candidate from UPO 4, UPO 5 and UPO 11 plates. We made minipreps with the day before inocula and analytic digestions. 0.8% agarosa gel analysis confirm 2+18 and 2+4+2+6.
We made colony PCR using colonies from 18+3 and 2+6+2+5+3 plates: no positive results.
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