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Team:UPO-Sevilla/Notebook/08 11
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<h2>Assembly Team</h2> | <h2>Assembly Team</h2> | ||
| - | <p>That day we got a lot of colonies red and white in at least every | + | <p>That day we got a lot of colonies red and white in at least every plate; non in: 12+19, 11+2+13+3 and 1+2+16+3 plaques. Non DNA controls were cleaned. We made colony PCR with 6 colonies from each plate. Agarose gel analysis showed than running time was too long. We could not rule out anything, so we set up inocula of each candidate.</p> |
<p>We set up inocula of positive candidate from UPO 4, UPO 5 and UPO 11 plates. We made minipreps with the day before inocula and analytic digestions. 0.8% agarosa gel analysis confirm 2+18 and 2+4+2+6.</p> | <p>We set up inocula of positive candidate from UPO 4, UPO 5 and UPO 11 plates. We made minipreps with the day before inocula and analytic digestions. 0.8% agarosa gel analysis confirm 2+18 and 2+4+2+6.</p> | ||
Revision as of 23:43, 26 October 2010
August, 11th
Assembly Team
That day we got a lot of colonies red and white in at least every plate; non in: 12+19, 11+2+13+3 and 1+2+16+3 plaques. Non DNA controls were cleaned. We made colony PCR with 6 colonies from each plate. Agarose gel analysis showed than running time was too long. We could not rule out anything, so we set up inocula of each candidate.
We set up inocula of positive candidate from UPO 4, UPO 5 and UPO 11 plates. We made minipreps with the day before inocula and analytic digestions. 0.8% agarosa gel analysis confirm 2+18 and 2+4+2+6.
We made colony PCR using colonies from 18+3 and 2+6+2+5+3 plates: no positive results.
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