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Team:UPO-Sevilla/Notebook/08 03
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<h2>Production Team</h2> | <h2>Production Team</h2> | ||
| - | <p><strong>Paola Gallardo.</strong> We digest <i>fecI-fecR</i> with restriction enzymes PstI and EcoRI, and run an electrophoresis. We purified from electrophoresis gel (1'5 | + | <p><strong>Paola Gallardo.</strong> We digest <i>fecI-fecR</i> with restriction enzymes PstI and EcoRI, and run an electrophoresis. We purified from electrophoresis gel (1'5 pb) and ligate it with the vector C3L (chloramphenicol).</p> |
<h2>Assembly Team</h2> | <h2>Assembly Team</h2> | ||
Revision as of 23:04, 26 October 2010
August, 3rd
Production Team
Paola Gallardo. We digest fecI-fecR with restriction enzymes PstI and EcoRI, and run an electrophoresis. We purified from electrophoresis gel (1'5 pb) and ligate it with the vector C3L (chloramphenicol).
Assembly Team
We analyzed several candidates from 2+4, 2+5 and 2+7 plates by colony PCR. We had positive candidates of UPO2+4 and UPO2+5. We set up inocula of these candidates and spread on LB+Tc plates.
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