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	== Blue Light Producer ==		== Blue Light Producer ==
	<br>		<br>
		+	'''5/7/2010'''
		+	*LuxAB & LuxCDE operon agarose gel
		+	* Lane1: About2.1.kbp
		+	* Lane 2: The highest band- very faint- about 3.5kbp
		+	[[Image:150.JPG]]
	'''13/7/2010'''		'''13/7/2010'''

---

Revision as of 22:51, 26 October 2010

Blue Light Producer

5/7/2010

• LuxAB & LuxCDE operon agarose gel
• Lane1: About2.1.kbp
• Lane 2: The highest band- very faint- about 3.5kbp

13/7/2010

Empty edinbrick vector for control.

• LuxAB + edinbrick ligation.

28/7/2010

an update on LuxAB:

• The transformations of Edinbrick are fine but all the luxABs failed (grew blue, not white on Xgal and did not glow, as of 27/7)/
• Still have some digested LuxAB-ediI so re-did ligation and transformation.

Lane 1&15	Lane 2	Others
Ladder	EdiI	LuxAB

GEL PIC

Lane 1	Lane 2 +3	Lanes 3-6
Ladder	EdiI	LuxAB

• The ligation had the same gel characteristics as previous luxAb-ediI ligations, e.x. it isn't visivle- not hopeful of this transformation working

29/7/2010

12/8/2010 update on LumP:

• still getting pink/red colonies from transformants
• single digests give a band of about 3kb
• double digests give a band of about 2.5 kb -highly confusing
• and also, no sign on RFP despite red/pink growth.
• Vector wa ssequenced and LumP is definately there.

gel pohot no x and y + description.

26/8/2010

• Marker (Lane 1), minipreps 6-10 luxABlumP (lanes 2-6)

• single digests of LuxABlumP

7/9/2010

• minipreps of luxCDE: samples 1&4 usable

Lane 1	Lane 2-6	Lanes 7
Ladder	luxCDE00:1-5 minipreps	Ladder