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Team:UPO-Sevilla/Notebook/08 03
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<h2>Assembly Team</h2> | <h2>Assembly Team</h2> | ||
| - | <p>Again colony PCR of six candidates: 2+4, 2+5 y 2+7. We have positive candidates of UPO2+4 and UPO2+5. We set up inocula of this candidates and spread | + | <p>Again colony PCR of six candidates: 2+4, 2+5 y 2+7. We have positive candidates of UPO2+4 and UPO2+5. We set up inocula of this candidates and spread on LB+Tc plates.</p> |
<a class="return_button" href="/Team:UPO-Sevilla/Notebook/08_02" title="Go to 2nd of August"><span>Go to 2nd of August</span></a> | <a class="return_button" href="/Team:UPO-Sevilla/Notebook/08_02" title="Go to 2nd of August"><span>Go to 2nd of August</span></a> | ||
Revision as of 22:48, 26 October 2010
August, 3rd
Production Team
Paola Gallardo. We digest fecI-fecR with restriction enzymes PstI and EcoRI, and run an electrophoresis. We purified from electrophoresis gel (1'5 pDC) and ligate it with the vector C3L (chloramphenicol).
Assembly Team
Again colony PCR of six candidates: 2+4, 2+5 y 2+7. We have positive candidates of UPO2+4 and UPO2+5. We set up inocula of this candidates and spread on LB+Tc plates.
Go to 2nd of August Go to 4th of August Return to Notebook
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