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Team:UPO-Sevilla/Notebook/07 27
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<p><strong>Paola Gallardo.</strong> We made the second round of the PCR reaction for the SDM, using suitable primers and fragments (gltD1+ gltD2, fecI-fecR1 + fecI-fecR2), and changing the characteristics of the cycles. Purification of the obtained products.</p> | <p><strong>Paola Gallardo.</strong> We made the second round of the PCR reaction for the SDM, using suitable primers and fragments (gltD1+ gltD2, fecI-fecR1 + fecI-fecR2), and changing the characteristics of the cycles. Purification of the obtained products.</p> | ||
| - | <p><strong>David Caballero.</strong> Inocula analysis of <i>fecI</i> and P<i>fecA</i>: vector purification and 1.2% agarose gel electrophoresis analysis. We had positive results for both | + | <p><strong>David Caballero.</strong> Inocula analysis of <i>fecI</i> and P<i>fecA</i>: vector purification and 1.2% agarose gel electrophoresis analysis. We had positive results for both parts. Two tests have been made to verify the obtaining of these new parts we added to the biobrick catalog: colony PCR and digestive purify vector analysis. We set up inocula of parts <i>gltB</i> and <i>fecI-fecR*</i> to do the second testing the next day. On the other hand we performed colony PCR reactions to test three colonies emerged in <i>fecA*</i> transformed plate. 0.8% agarose gel electrophoresis showed multiple lines, some of them coincident with <i>fecA*</i> size (2.4 kbp). So we set up inocula of these three new candidates and let them incubate overnight shaking at 37ºC.</p> |
<h2>DryLab Team</h2> | <h2>DryLab Team</h2> | ||
Latest revision as of 21:55, 26 October 2010
July, 27th
Production Team
Paola Gallardo. We made the second round of the PCR reaction for the SDM, using suitable primers and fragments (gltD1+ gltD2, fecI-fecR1 + fecI-fecR2), and changing the characteristics of the cycles. Purification of the obtained products.
David Caballero. Inocula analysis of fecI and PfecA: vector purification and 1.2% agarose gel electrophoresis analysis. We had positive results for both parts. Two tests have been made to verify the obtaining of these new parts we added to the biobrick catalog: colony PCR and digestive purify vector analysis. We set up inocula of parts gltB and fecI-fecR* to do the second testing the next day. On the other hand we performed colony PCR reactions to test three colonies emerged in fecA* transformed plate. 0.8% agarose gel electrophoresis showed multiple lines, some of them coincident with fecA* size (2.4 kbp). So we set up inocula of these three new candidates and let them incubate overnight shaking at 37ºC.
DryLab Team
Wiki: Overall wiki design completed. Some details to be concluded.
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