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Team:Imperial College London/Modelling/Signalling/Detailed Description
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|style="font-family: helvetica, arial, sans-serif;font-size:2em;color:#ea8828;"|Detailed Description | |style="font-family: helvetica, arial, sans-serif;font-size:2em;color:#ea8828;"|Detailed Description | ||
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Revision as of 21:46, 26 October 2010
| Modelling | Overview | Detection Model | Signaling Model | Fast Response Model | Interactions |
| A major part of the project consisted of modelling each module. This enabled us to decide which ideas we should implement. Look at the Fast Response page for a great example of how modelling has made a major impact on our design! | |
| Objectives | Description | Results | Constants | MATLAB Code |
| Detailed Description | ||
| We are assuming steady-state for AIP, Phosphate, ComD and ComE in our cell/in the solution. Hence, we can neglect production and degradation rates of ComD and ComE. | ||
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| Equation 1: AIP binds to ComD receptor to form a complex (AIP-ComD) | ||
| AIP + ComD ↔ AIP-ComD | ||
| Equation 2: Phosphate binds to the AIP-ComD complex to form another complex (AIP-ComD*) | ||
| AIP-ComD + Phosphate ↔ AIP-ComD* | ||
| Equation 3: ComE binds to the AIP-ComD* recepetor to form another complex (AIP-ComD*-ComE) | ||
| AIP-ComD* + ComE ↔ AIP-ComD*-ComE | ||
| Equation 4: Phosphate group on ComD binds to ComE and forms two products: phosphorylated ComE (ComE*) and AIP-ComD | ||
| AIP-ComD*-ComE ↔ AIP-ComD + ComE* | ||
| Using the Law of Mass Action, we can rewrite these 4 equations: | ||
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| In the above equations, the forwards reaction constants are represented by k1,2,3,4, for the first, second, third and fourth reaction, respectively. The backwards reaction constants are represented by k-1,-2,-3,-4. |
