"
Team:TU Delft/30 June 2010 content
From 2010.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| + | ====Lab Work=== | ||
| + | |||
We harvested the 50 mL bacterial cells of the 15 biobricks from the distribution plates. We used 3 mL of the baceterial cells to make [[Team:TU_Delft/protocols/freezing_bacterial_stocks|-80 °C stocks]]. With the rest we performed a [[Team:TU_Delft/protocols/midi-prep_plasmid_isolation|Qiagen Midi-prep plasmid isolation]]. | We harvested the 50 mL bacterial cells of the 15 biobricks from the distribution plates. We used 3 mL of the baceterial cells to make [[Team:TU_Delft/protocols/freezing_bacterial_stocks|-80 °C stocks]]. With the rest we performed a [[Team:TU_Delft/protocols/midi-prep_plasmid_isolation|Qiagen Midi-prep plasmid isolation]]. | ||
At the same time we are making more competent cells. We go through our competent cells so quickly. | At the same time we are making more competent cells. We go through our competent cells so quickly. | ||
Revision as of 12:39, 7 July 2010
=Lab Work
We harvested the 50 mL bacterial cells of the 15 biobricks from the distribution plates. We used 3 mL of the baceterial cells to make -80 °C stocks. With the rest we performed a Qiagen Midi-prep plasmid isolation. At the same time we are making more competent cells. We go through our competent cells so quickly.
