Team:BCCS-Bristol/Wetlab

From 2010.igem.org

(Difference between revisions)
(→Safety Issues- From 2009, consider deleting)
Line 1: Line 1:
{{:Team:BCCS-Bristol/Header}}
{{:Team:BCCS-Bristol/Header}}
 +
 +
 +
== Wet lab ==
 +
 +
[[Image:Picture_018.jpg|right|200px|Oli's labspace]]
 +
 +
Our main achievement in the wet lab has been the creation of a new composite biobrick, engineering ''E. coli'' to produce GFP in response to Nitrates. Not only is this part complete, but it's also well characterised both on its own and working in tandem with a second biobrick.
 +
 +
We also developed a way of encapsulating our bacteria, allowing not only an easy and safe method of spreading them in the environment, but also dramatically improving signal detection and ''E. coli'' survival in soil.
 +
 +
Finally we worked to further characterise the PyeaR promoter submitted by Edinburgh in 2009, improving the quality of information available in the parts registry.
 +
 +
 +
In this section you can find out about the design of our parts, including all the decisions we made and why, as well as all the information relating to their construction and characterisation. It also contains material on our new encapsulation methods, and finally lists all our safety considerations both in the lab and on a wider environmental scale.
 +
 +
For a more in depth view of what we have done so far, click on the [https://2010.igem.org/Team:BCCS-Bristol/Wetlab/Notebook Notebook] section.
 +
 +
 +
For a complete list of the biobricks used during our project, plus a brief description on their use, click [https://2010.igem.org/Team:BCCS-Bristol/Wetlab/BioBricks here].
 +
==Subpage Structure==
==Subpage Structure==
Line 24: Line 44:
[[:Team:BCCS-Bristol/Wetlab/Lab_photos]]
[[:Team:BCCS-Bristol/Wetlab/Lab_photos]]
-
 
-
== Wet lab ==
 
-
 
-
[[Image:Picture_018.jpg|right|200px|Oli's labspace]]
 
-
 
-
The wet lab team have been focussing on further characterising the PyeaR promoter submitted by Edinburgh last year and on creating our own new Biobricks to engineer E. coli to produce GFP in response to nitrates.
 
-
 
-
 
-
We have also been examining different methods of encapsulating our bacteria to create pellets that could be easily spread by farmers on fields, as well as looking at signal detection and E. coli survival on soil.
 
-
 
-
 
-
For a more in depth view of what we have done so far, click on the [https://2010.igem.org/Team:BCCS-Bristol/Wetlab/Notebook Notebook] section.
 
-
 
-
 
-
For a complete list of the biobricks used during our project, plus a brief description on their use, click [https://2010.igem.org/Team:BCCS-Bristol/Wetlab/BioBricks here].
 
-
 
-
 

Revision as of 20:08, 26 October 2010


Wet lab

Oli's labspace

Our main achievement in the wet lab has been the creation of a new composite biobrick, engineering E. coli to produce GFP in response to Nitrates. Not only is this part complete, but it's also well characterised both on its own and working in tandem with a second biobrick.

We also developed a way of encapsulating our bacteria, allowing not only an easy and safe method of spreading them in the environment, but also dramatically improving signal detection and E. coli survival in soil.

Finally we worked to further characterise the PyeaR promoter submitted by Edinburgh in 2009, improving the quality of information available in the parts registry.


In this section you can find out about the design of our parts, including all the decisions we made and why, as well as all the information relating to their construction and characterisation. It also contains material on our new encapsulation methods, and finally lists all our safety considerations both in the lab and on a wider environmental scale.

For a more in depth view of what we have done so far, click on the Notebook section.


For a complete list of the biobricks used during our project, plus a brief description on their use, click here.


Subpage Structure

Team:BCCS-Bristol/Wetlab/Part_Design

Team:BCCS-Bristol/Wetlab/Beads

Team:BCCS-Bristol/Wetlab/Improvements

Team:BCCS-Bristol/Wetlab/Experiments

Team:BCCS-Bristol/Wetlab/Safety

Team:BCCS-Bristol/Wetlab/Project_Results

Team:BCCS-Bristol/Wetlab/Image_Gallery

Links to old pages

Team:BCCS-Bristol/Wetlab/Notebook

Team:BCCS-Bristol/Wetlab/BioBricks

Team:BCCS-Bristol/Wetlab/Lab_photos



Retrieved from "http://2010.igem.org/Team:BCCS-Bristol/Wetlab"