Team:Lethbridge/Project/Catechol Degradation
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=<font color="white"> Catechol Degradation= | =<font color="white"> Catechol Degradation= | ||
- | Our home province, Alberta, has proven oil reserves of 171.3 billion barrels. These reserves make up the second-largest proven crude oil reserve in the world. This is enough oil to meet Canada’s current oil demand for almost 400 years<sup>1</sup>. The oil reserves are in the form of tar sands: a sand, clay, water, and bitumen mixture< | + | Our home province, Alberta, has proven oil reserves of 171.3 billion barrels. These reserves make up the second-largest proven crude oil reserve in the world. This is enough oil to meet Canada’s current oil demand for almost 400 years<sup>1</sup>. The oil reserves are in the form of tar sands: a sand, clay, water, and bitumen mixture<sup>2</sup>. The bitumen is separated from the sticky tar sand by washing it with hot water. The heated water acquires toxins from the tar sands: resulting in an environmental issue. |
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Catechol is a toxic organic molecule commonly found in tailings ponds. Furthermore, many other toxic compounds, such as naphthenic acids, can be metabolized into catechol. <i>Pseudomonas putida</i> demonstrates great metabolic diversity and is able to utilize a wide range of carbon sources, including molecules few other organisms can break down<sup>3</sup>. The <html><a href="https://2008.igem.org/Team:University_of_Lethbridge" target="new"><font color="green"> Lethbridge 2008 iGEM</font></a></html> isolated the <i>XylE</i> gene (<html><a href="http://partsregistry.org/Part:BBa_K147002" target="new"><font color="green">BBa_K147002</font></a></html>) from <i>P.putida</i>, which codes for catechol 2,3-dioxygenase (XylE). However, the <html><a href="https://2008.igem.org/Team:Edinburgh" target="new"><font color="green">Edinburgh 2008 iGEM</font></a></html> team isolated the <i>XylE</i> gene (<html><a href="http://partsregistry.org/Part:BBa_K118021" target="new"><font color="green">BBa_K118021</font></a></html>) from <i>P.putida</i>, along with a ribosomal binding site (rbs), so we chose to work with this part. This year we have engineered <i>Escherichia coli</i> DH5αto express XylE. XylE rapidly converts catechol into 2-hydroxymuconic semialdehyde (2-HMS). 2-HMS is a non-toxic, bright yellow molecule that can be metalbolized by <i>E.coli</i> DH5α. | Catechol is a toxic organic molecule commonly found in tailings ponds. Furthermore, many other toxic compounds, such as naphthenic acids, can be metabolized into catechol. <i>Pseudomonas putida</i> demonstrates great metabolic diversity and is able to utilize a wide range of carbon sources, including molecules few other organisms can break down<sup>3</sup>. The <html><a href="https://2008.igem.org/Team:University_of_Lethbridge" target="new"><font color="green"> Lethbridge 2008 iGEM</font></a></html> isolated the <i>XylE</i> gene (<html><a href="http://partsregistry.org/Part:BBa_K147002" target="new"><font color="green">BBa_K147002</font></a></html>) from <i>P.putida</i>, which codes for catechol 2,3-dioxygenase (XylE). However, the <html><a href="https://2008.igem.org/Team:Edinburgh" target="new"><font color="green">Edinburgh 2008 iGEM</font></a></html> team isolated the <i>XylE</i> gene (<html><a href="http://partsregistry.org/Part:BBa_K118021" target="new"><font color="green">BBa_K118021</font></a></html>) from <i>P.putida</i>, along with a ribosomal binding site (rbs), so we chose to work with this part. This year we have engineered <i>Escherichia coli</i> DH5αto express XylE. XylE rapidly converts catechol into 2-hydroxymuconic semialdehyde (2-HMS). 2-HMS is a non-toxic, bright yellow molecule that can be metalbolized by <i>E.coli</i> DH5α. |