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Team:WITS-South Africa/Machine Design
From 2010.igem.org
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Purpose: | Purpose: | ||
| - | a) To show that the PlcR promoter is activated in ''L. gasseri'' by measuring fluorescence after the addition of exogenous PlcR and PapR | + | a) To show that the PlcR promoter is activated in ''L. gasseri'' by measuring fluorescence after the addition of exogenous PlcR and PapR proteins |
| - | [[Image: | + | [[Image:Wits_Lacto-test.jpg]] |
===Machine Testing=== | ===Machine Testing=== | ||
Revision as of 19:34, 26 October 2010
Contents |
Lacto-detect
Purpose:
a) To act as the ‘Detector’ Machine within the population and produce the quorum signalling peptide in response to an input signal
So how did we end up selecting the final parts of our Machines? Each one was chosen after much consideration and scouring of the literature to select the most suitable biological system. For a design rationale of why we selected the parts that we did to create this machine, click here
For an account of how we assemblied and verified Lacto-detect, click here
Lacto-report
For a design rationale of why we selected the parts that we did to create this machine, click here
We were not able to finish constructing Lacto-report in it's entirety, due to time constraints and issues with the E.chromi Biobrick parts. (For more details, click here.)
For testing and characterising the behaviour of the quorum sensing mechanism in a model Gram-positive bacillus, we used an intermediate machine construct we dubbed Lacto-
Lacto-test
Purpose:
a) To show that the PlcR promoter is activated in L. gasseri by measuring fluorescence after the addition of exogenous PlcR and PapR proteins
