Team:Uppsala-SwedenWeek15

From 2010.igem.org

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{{Template:Uppsala}}<!--Do not remove the first and last lines in this page!-->
{{Template:Uppsala}}<!--Do not remove the first and last lines in this page!-->
== Week-15 ==
== Week-15 ==
 +
 +
== Plasmid concentration of K1, K2, K3, (old version) and digestion ==
 +
 +
 +
<TABLE WIDTH=617 BORDER=1 BORDERCOLOR="#000000" CELLPADDING=7 CELLSPACING=0>
 +
<COL WIDTH=83>
 +
<COL WIDTH=84>
 +
<COL WIDTH=84>
 +
<COL WIDTH=99>
 +
<COL WIDTH=70>
 +
<COL WIDTH=34>
 +
<COL WIDTH=61>
 +
<TR VALIGN=TOP>
 +
<TD WIDTH=83>
 +
<P LANG="sv-SE" CLASS="western">Sample</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western" STYLE="margin-bottom: 0in">DNA-concentration</P>
 +
<P LANG="sv-SE" CLASS="western">(µL/ml)</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">DNA
 +
</P>
 +
</TD>
 +
<TD WIDTH=99>
 +
<P LANG="sv-SE" CLASS="western">Enzymes (E &amp; P)</P>
 +
</TD>
 +
<TD WIDTH=70>
 +
<P LANG="sv-SE" CLASS="western">FDB (10X)</P>
 +
</TD>
 +
<TD WIDTH=34>
 +
<P LANG="sv-SE" CLASS="western">H20</P>
 +
</TD>
 +
<TD WIDTH=61>
 +
<P LANG="sv-SE" CLASS="western">Total (µL)</P>
 +
</TD>
 +
</TR>
 +
<TR VALIGN=TOP>
 +
<TD WIDTH=83>
 +
<P LANG="sv-SE" CLASS="western">K1C1</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">32,7</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">15.3</P>
 +
</TD>
 +
<TD WIDTH=99>
 +
<P LANG="sv-SE" CLASS="western" ALIGN=CENTER>0,5 &amp;0.5</P>
 +
</TD>
 +
<TD WIDTH=70>
 +
<P LANG="sv-SE" CLASS="western">2</P>
 +
</TD>
 +
<TD WIDTH=34>
 +
<P LANG="sv-SE" CLASS="western">1.7</P>
 +
</TD>
 +
<TD WIDTH=61>
 +
<P LANG="sv-SE" CLASS="western">20</P>
 +
</TD>
 +
</TR>
 +
<TR VALIGN=TOP>
 +
<TD WIDTH=83>
 +
<P LANG="sv-SE" CLASS="western">K1C2</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">98.3</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">5.0</P>
 +
</TD>
 +
<TD WIDTH=99>
 +
<P LANG="sv-SE" CLASS="western" ALIGN=CENTER>0,5 &amp;0.5</P>
 +
</TD>
 +
<TD WIDTH=70>
 +
<P LANG="sv-SE" CLASS="western">2</P>
 +
</TD>
 +
<TD WIDTH=34>
 +
<P LANG="sv-SE" CLASS="western">12</P>
 +
</TD>
 +
<TD WIDTH=61>
 +
<P LANG="sv-SE" CLASS="western">20</P>
 +
</TD>
 +
</TR>
 +
<TR VALIGN=TOP>
 +
<TD WIDTH=83>
 +
<P LANG="sv-SE" CLASS="western">K1C3</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">74.4</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">6.7</P>
 +
</TD>
 +
<TD WIDTH=99>
 +
<P LANG="sv-SE" CLASS="western" ALIGN=CENTER>0,5 &amp;0.5</P>
 +
</TD>
 +
<TD WIDTH=70>
 +
<P LANG="sv-SE" CLASS="western">2</P>
 +
</TD>
 +
<TD WIDTH=34>
 +
<P LANG="sv-SE" CLASS="western">10.3</P>
 +
</TD>
 +
<TD WIDTH=61>
 +
<P LANG="sv-SE" CLASS="western">20</P>
 +
</TD>
 +
</TR>
 +
<TR VALIGN=TOP>
 +
<TD WIDTH=83>
 +
<P LANG="sv-SE" CLASS="western">K1C4</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">516.4</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">0.9</P>
 +
</TD>
 +
<TD WIDTH=99>
 +
<P LANG="sv-SE" CLASS="western" ALIGN=CENTER>0,5 &amp;0.5</P>
 +
</TD>
 +
<TD WIDTH=70>
 +
<P LANG="sv-SE" CLASS="western">1</P>
 +
</TD>
 +
<TD WIDTH=34>
 +
<P LANG="sv-SE" CLASS="western">7.1</P>
 +
</TD>
 +
<TD WIDTH=61>
 +
<P LANG="sv-SE" CLASS="western">10</P>
 +
</TD>
 +
</TR>
 +
<TR VALIGN=TOP>
 +
<TD WIDTH=83>
 +
<P LANG="sv-SE" CLASS="western">K1C5</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">135.4</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">3.6</P>
 +
</TD>
 +
<TD WIDTH=99>
 +
<P LANG="sv-SE" CLASS="western" ALIGN=CENTER>0,5 &amp;0.5</P>
 +
</TD>
 +
<TD WIDTH=70>
 +
<P LANG="sv-SE" CLASS="western">1</P>
 +
</TD>
 +
<TD WIDTH=34>
 +
<P LANG="sv-SE" CLASS="western">4.4</P>
 +
</TD>
 +
<TD WIDTH=61>
 +
<P LANG="sv-SE" CLASS="western">10</P>
 +
</TD>
 +
</TR>
 +
<TR VALIGN=TOP>
 +
<TD WIDTH=83>
 +
<P LANG="sv-SE" CLASS="western">K2C1</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">161.7</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">3.1</P>
 +
</TD>
 +
<TD WIDTH=99>
 +
<P LANG="sv-SE" CLASS="western" ALIGN=CENTER>0,5 &amp;0.5</P>
 +
</TD>
 +
<TD WIDTH=70>
 +
<P LANG="sv-SE" CLASS="western">1</P>
 +
</TD>
 +
<TD WIDTH=34>
 +
<P LANG="sv-SE" CLASS="western">4.9</P>
 +
</TD>
 +
<TD WIDTH=61>
 +
<P LANG="sv-SE" CLASS="western">10</P>
 +
</TD>
 +
</TR>
 +
<TR VALIGN=TOP>
 +
<TD WIDTH=83>
 +
<P LANG="sv-SE" CLASS="western">K2C2</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">92.3</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">5.4</P>
 +
</TD>
 +
<TD WIDTH=99>
 +
<P LANG="sv-SE" CLASS="western" ALIGN=CENTER>0,5 &amp;0.5</P>
 +
</TD>
 +
<TD WIDTH=70>
 +
<P LANG="sv-SE" CLASS="western">1</P>
 +
</TD>
 +
<TD WIDTH=34>
 +
<P LANG="sv-SE" CLASS="western">2.6</P>
 +
</TD>
 +
<TD WIDTH=61>
 +
<P LANG="sv-SE" CLASS="western">10</P>
 +
</TD>
 +
</TR>
 +
<TR VALIGN=TOP>
 +
<TD WIDTH=83>
 +
<P LANG="sv-SE" CLASS="western">K2C3</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">112.2</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">4.3</P>
 +
</TD>
 +
<TD WIDTH=99>
 +
<P LANG="sv-SE" CLASS="western" ALIGN=CENTER>0,5 &amp;0.5</P>
 +
</TD>
 +
<TD WIDTH=70>
 +
<P LANG="sv-SE" CLASS="western">1</P>
 +
</TD>
 +
<TD WIDTH=34>
 +
<P LANG="sv-SE" CLASS="western">3.7</P>
 +
</TD>
 +
<TD WIDTH=61>
 +
<P LANG="sv-SE" CLASS="western">10</P>
 +
</TD>
 +
</TR>
 +
<TR VALIGN=TOP>
 +
<TD WIDTH=83>
 +
<P LANG="sv-SE" CLASS="western">K2C4</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">45.6</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">10.9</P>
 +
</TD>
 +
<TD WIDTH=99>
 +
<P LANG="sv-SE" CLASS="western" ALIGN=CENTER>0,5 &amp;0.5</P>
 +
</TD>
 +
<TD WIDTH=70>
 +
<P LANG="sv-SE" CLASS="western">2</P>
 +
</TD>
 +
<TD WIDTH=34>
 +
<P LANG="sv-SE" CLASS="western">6.1</P>
 +
</TD>
 +
<TD WIDTH=61>
 +
<P LANG="sv-SE" CLASS="western">20</P>
 +
</TD>
 +
</TR>
 +
<TR VALIGN=TOP>
 +
<TD WIDTH=83>
 +
<P LANG="sv-SE" CLASS="western">K2C5</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">348.1</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">1.4</P>
 +
</TD>
 +
<TD WIDTH=99>
 +
<P LANG="sv-SE" CLASS="western" ALIGN=CENTER>0,5 &amp;0.5</P>
 +
</TD>
 +
<TD WIDTH=70>
 +
<P LANG="sv-SE" CLASS="western">1</P>
 +
</TD>
 +
<TD WIDTH=34>
 +
<P LANG="sv-SE" CLASS="western">6.6</P>
 +
</TD>
 +
<TD WIDTH=61>
 +
<P LANG="sv-SE" CLASS="western">10</P>
 +
</TD>
 +
</TR>
 +
<TR VALIGN=TOP>
 +
<TD WIDTH=83>
 +
<P LANG="sv-SE" CLASS="western">K2C6</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">91.3</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">5.4</P>
 +
</TD>
 +
<TD WIDTH=99>
 +
<P LANG="sv-SE" CLASS="western" ALIGN=CENTER>0,5 &amp;0.5</P>
 +
</TD>
 +
<TD WIDTH=70>
 +
<P LANG="sv-SE" CLASS="western">1</P>
 +
</TD>
 +
<TD WIDTH=34>
 +
<P LANG="sv-SE" CLASS="western">2.6</P>
 +
</TD>
 +
<TD WIDTH=61>
 +
<P LANG="sv-SE" CLASS="western">10</P>
 +
</TD>
 +
</TR>
 +
<TR VALIGN=TOP>
 +
<TD WIDTH=83>
 +
<P LANG="sv-SE" CLASS="western">K3C1</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">88.4</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">5.6</P>
 +
</TD>
 +
<TD WIDTH=99>
 +
<P LANG="sv-SE" CLASS="western" ALIGN=CENTER>0,5 &amp;0.5</P>
 +
</TD>
 +
<TD WIDTH=70>
 +
<P LANG="sv-SE" CLASS="western">1</P>
 +
</TD>
 +
<TD WIDTH=34>
 +
<P LANG="sv-SE" CLASS="western">2.4</P>
 +
</TD>
 +
<TD WIDTH=61>
 +
<P LANG="sv-SE" CLASS="western">10</P>
 +
</TD>
 +
</TR>
 +
<TR VALIGN=TOP>
 +
<TD WIDTH=83>
 +
<P LANG="sv-SE" CLASS="western">K3C2</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">109.9</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">4.5</P>
 +
</TD>
 +
<TD WIDTH=99>
 +
<P LANG="sv-SE" CLASS="western" ALIGN=CENTER>0,5 &amp;0.5</P>
 +
</TD>
 +
<TD WIDTH=70>
 +
<P LANG="sv-SE" CLASS="western">1</P>
 +
</TD>
 +
<TD WIDTH=34>
 +
<P LANG="sv-SE" CLASS="western">3.5</P>
 +
</TD>
 +
<TD WIDTH=61>
 +
<P LANG="sv-SE" CLASS="western">10</P>
 +
</TD>
 +
</TR>
 +
<TR VALIGN=TOP>
 +
<TD WIDTH=83>
 +
<P LANG="sv-SE" CLASS="western">K3C3</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">116.6</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">4.3</P>
 +
</TD>
 +
<TD WIDTH=99>
 +
<P LANG="sv-SE" CLASS="western" ALIGN=CENTER>0,5 &amp;0.5</P>
 +
</TD>
 +
<TD WIDTH=70>
 +
<P LANG="sv-SE" CLASS="western">1</P>
 +
</TD>
 +
<TD WIDTH=34>
 +
<P LANG="sv-SE" CLASS="western">3.8</P>
 +
</TD>
 +
<TD WIDTH=61>
 +
<P LANG="sv-SE" CLASS="western">10</P>
 +
</TD>
 +
</TR>
 +
<TR VALIGN=TOP>
 +
<TD WIDTH=83>
 +
<P LANG="sv-SE" CLASS="western">K3C4</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">110.0</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">4.6</P>
 +
</TD>
 +
<TD WIDTH=99>
 +
<P LANG="sv-SE" CLASS="western" ALIGN=CENTER>0,5 &amp;0.5</P>
 +
</TD>
 +
<TD WIDTH=70>
 +
<P LANG="sv-SE" CLASS="western">1</P>
 +
</TD>
 +
<TD WIDTH=34>
 +
<P LANG="sv-SE" CLASS="western">3.5</P>
 +
</TD>
 +
<TD WIDTH=61>
 +
<P LANG="sv-SE" CLASS="western">10</P>
 +
</TD>
 +
</TR>
 +
<TR VALIGN=TOP>
 +
<TD WIDTH=83>
 +
<P LANG="sv-SE" CLASS="western">K3C5</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">90.67</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">5.5</P>
 +
</TD>
 +
<TD WIDTH=99>
 +
<P LANG="sv-SE" CLASS="western" ALIGN=CENTER>0,5 &amp;0.5</P>
 +
</TD>
 +
<TD WIDTH=70>
 +
<P LANG="sv-SE" CLASS="western">1</P>
 +
</TD>
 +
<TD WIDTH=34>
 +
<P LANG="sv-SE" CLASS="western">2.5</P>
 +
</TD>
 +
<TD WIDTH=61>
 +
<P LANG="sv-SE" CLASS="western">10</P>
 +
</TD>
 +
</TR>
 +
<TR VALIGN=TOP>
 +
<TD WIDTH=83>
 +
<P LANG="sv-SE" CLASS="western">K3C6</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">109.5</P>
 +
</TD>
 +
<TD WIDTH=84>
 +
<P LANG="sv-SE" CLASS="western">4.6</P>
 +
</TD>
 +
<TD WIDTH=99>
 +
<P LANG="sv-SE" CLASS="western" ALIGN=CENTER>0,5 &amp;0.5</P>
 +
</TD>
 +
<TD WIDTH=70>
 +
<P LANG="sv-SE" CLASS="western">1</P>
 +
</TD>
 +
<TD WIDTH=34>
 +
<P LANG="sv-SE" CLASS="western">3.4</P>
 +
</TD>
 +
<TD WIDTH=61>
 +
<P LANG="sv-SE" CLASS="western">10</P>
 +
</TD>
 +
</TR>
 +
</TABLE>

Revision as of 19:19, 26 October 2010

Week-15

Plasmid concentration of K1, K2, K3, (old version) and digestion

<COL WIDTH=83> <COL WIDTH=84> <COL WIDTH=84> <COL WIDTH=99> <COL WIDTH=70> <COL WIDTH=34> <COL WIDTH=61>

Sample

DNA-concentration

(µL/ml)

DNA

Enzymes (E & P)

FDB (10X)

H20

Total (µL)

K1C1

32,7

15.3

0,5 &0.5

2

1.7

20

K1C2

98.3

5.0

0,5 &0.5

2

12

20

K1C3

74.4

6.7

0,5 &0.5

2

10.3

20

K1C4

516.4

0.9

0,5 &0.5

1

7.1

10

K1C5

135.4

3.6

0,5 &0.5

1

4.4

10

K2C1

161.7

3.1

0,5 &0.5

1

4.9

10

K2C2

92.3

5.4

0,5 &0.5

1

2.6

10

K2C3

112.2

4.3

0,5 &0.5

1

3.7

10

K2C4

45.6

10.9

0,5 &0.5

2

6.1

20

K2C5

348.1

1.4

0,5 &0.5

1

6.6

10

K2C6

91.3

5.4

0,5 &0.5

1

2.6

10

K3C1

88.4

5.6

0,5 &0.5

1

2.4

10

K3C2

109.9

4.5

0,5 &0.5

1

3.5

10

K3C3

116.6

4.3

0,5 &0.5

1

3.8

10

K3C4

110.0

4.6

0,5 &0.5

1

3.5

10

K3C5

90.67

5.5

0,5 &0.5

1

2.5

10

K3C6

109.5

4.6

0,5 &0.5

1

3.4

10
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