Team:Alberta/Notebook/protocols/gel extraction

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Revision as of 17:07, 26 October 2010

TEAM ALBERTA

Protocol 7: Gel Extraction


Reagents:

  • Clean scalpel
  • Transilluminator
  • Eppendorf tube
  • Gel extraction kit
  • 55oC water bath
  • Vortex
  • Microfuge


Procedure:

  • Place the gel on the transilluminator. Put on face shield and gloves.
  • Turn on transilluminator and quickly make four slices; one on each side of the band you want to cut out and turn transilluminator off. Keep exposure to a minimum.
  • Pry out the gel ‘cube’ and place it in an eppendorf tube.
  • Follow instructions on pp. 25 of [http://www.qiagen.com/literature/render.aspx?id=103715 QIAQuick Spin Handbook].


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