Team:Alberta/Notebook/protocols/glycerol

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Revision as of 17:05, 26 October 2010

TEAM ALBERTA

Protocol 3: Glycerol Stock


Reagents:

  • Overnight with bacterial growth (probably 1.5mL)
  • Screw cap tubes
  • Glycerol


Procedure:

  • Pipet 150uL 50% glycerol into 3* properly labeled 1.5 ml screw cap tubes.
  • Add 350uL of overnight culture to each tube.
  • Pipet up and down to gently mix.
  • Flash freeze in liquid N2 or dry ice/methanol bath.
  • Place in -80oC freezer when frozen.


Notes:

  • If we make extra we have them if we need to send them out or want to store in several locations.


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