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Team:TU Delft/30 June 2010 content
From 2010.igem.org
(Difference between revisions)
(New page: We harvested the 50 mL bacterial cells of the 15 biobricks from the distribution plates. We used 3 mL of the baceterial cells to make -80 °C stocks. With the rest we performed a Qiagen Mi...) |
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| - | We harvested the 50 mL bacterial cells of the 15 biobricks from the distribution plates. We used 3 mL of the baceterial cells to make -80 °C stocks. With the rest we performed a Qiagen Midi-prep plasmid isolation. | + | We harvested the 50 mL bacterial cells of the 15 biobricks from the distribution plates. We used 3 mL of the baceterial cells to make [[Team:TU_Delft/protocols/freezing_bacterial_stocks|-80 °C stocks]]. With the rest we performed a [[Team:TU_Delft/protocols/midi-prep_plasmid_isolation|Qiagen Midi-prep plasmid isolation]]. |
| - | At the same time we are making more competent cells. We go through our competent cells so quickly | + | At the same time we are making more competent cells. We go through our competent cells so quickly. |
Revision as of 10:13, 6 July 2010
We harvested the 50 mL bacterial cells of the 15 biobricks from the distribution plates. We used 3 mL of the baceterial cells to make -80 °C stocks. With the rest we performed a Qiagen Midi-prep plasmid isolation. At the same time we are making more competent cells. We go through our competent cells so quickly.
