Team:Tsinghua/experiments
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PCR: Amplication eGFP, mCherry, Kanamycin resistant gene and Chlr four genes separatly from PIB-eGFP, PBS34, PKD13 and PKD3 plasmids. | PCR: Amplication eGFP, mCherry, Kanamycin resistant gene and Chlr four genes separatly from PIB-eGFP, PBS34, PKD13 and PKD3 plasmids. | ||
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[[Image:Fan2.jpg|after the gel extraction]] | [[Image:Fan2.jpg|after the gel extraction]] |
Revision as of 11:28, 26 October 2010
Experiment Records
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Our Experiments were carried out by the following nine groups which are somewhat independent from each other. Here come the Groups and Their Tasks.
Group 1A:Landing pad (LP) construction and Insertion Project with Att Recomination method and Trandition Landing pad
Protocols
Molecular CloningTHU_Protocol_1-1_Isolation_of_plasmid_DNA
THU_Protocol_1-3_Restriction_Enzyme_Digestion
THU_Protocol_1-4_Ligation_of_DNA_Fragments
THU_Protocol_1-10_Electro_Transformation_of_Recombinant_DNA
THU_Protocol_1-15_PCR
Protein Isolation and Identification
Results
Group 2(b)PCR: Amplication eGFP, mCherry, Kanamycin resistant gene and Chlr four genes separatly from PIB-eGFP, PBS34, PKD13 and PKD3 plasmids.
after the gel extraction
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