Team:Tsinghua/experiments

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PCR: Amplication eGFP, mCherry, Kanamycin resistant gene and Chlr four genes separatly from PIB-eGFP, PBS34, PKD13 and PKD3 plasmids.  
PCR: Amplication eGFP, mCherry, Kanamycin resistant gene and Chlr four genes separatly from PIB-eGFP, PBS34, PKD13 and PKD3 plasmids.  
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[[Image:Fan1.JPG]]
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[[Image:Fan1]]
[[Image:Fan1.JPG]]
[[Image:Fan1.JPG]]
[[Image:Fan2.jpg|after the gel extraction]]
[[Image:Fan2.jpg|after the gel extraction]]

Revision as of 11:23, 26 October 2010

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Experiment Records

July
MTWTFSS
      [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/1_July_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/2_July_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/3_July_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/4_July_2010&action=edit 4]
[http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/5_July_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/6_July_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/7_July_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/8_July_2010&action=edit 8] [http://2010.igem.org/Team:Tsinghua/Notebook/9_July_2010 9] [http://2010.igem.org/Team:Tsinghua/Notebook/10_July_2010 10] [http://2010.igem.org/Team:Tsinghua/Notebook/11_July_2010 11]
[http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/12_July_2010&action=edit 12] [http://2010.igem.org/Team:Tsinghua/Notebook/13_July_2010 13] [http://2010.igem.org/Team:Tsinghua/Notebook/14_July_2010 14] [http://2010.igem.org/Team:Tsinghua/Notebook/15_July_2010 15] [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/16_July_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/17_July_2010&action=edit 17] [http://2010.igem.org/Team:Tsinghua/Notebook/18_July_2010 18]
[http://2010.igem.org/Team:Tsinghua/Notebook/19_July_2010 19] [http://2010.igem.org/Team:Tsinghua/Notebook/20_July_2010 20] [http://2010.igem.org/Team:Tsinghua/Notebook/21_July_2010 21] [http://2010.igem.org/Team:Tsinghua/Notebook/22_July_2010 22] [http://2010.igem.org/Team:Tsinghua/Notebook/23_July_2010 23] [http://2010.igem.org/Team:Tsinghua/Notebook/24_July_2010 24] [http://2010.igem.org/Team:Tsinghua/Notebook/25_July_2010 25]
[http://2010.igem.org/Team:Tsinghua/Notebook/26_July_2010 26] [http://2010.igem.org/Team:Tsinghua/Notebook/27_July_2010 27] [http://2010.igem.org/Team:Tsinghua/Notebook/28_July_2010 28] [http://2010.igem.org/Team:Tsinghua/Notebook/29_July_2010 29] [http://2010.igem.org/Team:Tsinghua/Notebook/30_July_2010 30] [http://2010.igem.org/Team:Tsinghua/Notebook/31_July_2010 31]
August
MTWTFSS
            [http://2010.igem.org/Team:Tsinghua/Notebook/1_August_2010 1]
[http://2010.igem.org/Team:Tsinghua/Notebook/2_August_2010 2] [http://2010.igem.org/Team:Tsinghua/Notebook/3_August_2010 3] [http://2010.igem.org/Team:Tsinghua/Notebook/4_August_2010 4] [http://2010.igem.org/Team:Tsinghua/Notebook/5_August_2010 5] [http://2010.igem.org/Team:Tsinghua/Notebook/6_August_2010 6] [http://2010.igem.org/Team:Tsinghua/Notebook/7_August_2010 7] [http://2010.igem.org/Team:Tsinghua/Notebook/8_August_2010 8]
[http://2010.igem.org/Team:Tsinghua/Notebook/9_August_2010 9] [http://2010.igem.org/Team:Tsinghua/Notebook/10_August_2010 10] [http://2010.igem.org/Team:Tsinghua/Notebook/11_August_2010 11] [http://2010.igem.org/Team:Tsinghua/Notebook/12_August_2010 12] [http://2010.igem.org/Team:Tsinghua/Notebook/13_August_2010 13] [http://2010.igem.org/Team:Tsinghua/Notebook/14_August_2010 14] [http://2010.igem.org/Team:Tsinghua/Notebook/15_August_2010 15]
[http://2010.igem.org/Team:Tsinghua/Notebook/16_August_2010 16] [http://2010.igem.org/Team:Tsinghua/Notebook/17_August_2010 17] [http://2010.igem.org/Team:Tsinghua/Notebook/18_August_2010 18] [http://2010.igem.org/Team:Tsinghua/Notebook/19_August_2010 19] [http://2010.igem.org/Team:Tsinghua/Notebook/20_August_2010 20] [http://2010.igem.org/Team:Tsinghua/Notebook/21_August_2010 21] [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/22_August_2010&action=edit 22]
[http://2010.igem.org/Team:Tsinghua/Notebook/23_August_2010 23] [http://2010.igem.org/Team:Tsinghua/Notebook/24_August_2010 24] [http://2010.igem.org/Team:Tsinghua/Notebook/25_August_2010 25] [http://2010.igem.org/Team:Tsinghua/Notebook/26_August_2010 26] [http://2010.igem.org/Team:Tsinghua/Notebook/27_August_2010 27] [http://2010.igem.org/Team:Tsinghua/Notebook/28_August_2010 28] [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/29_August_2010&action=edit 29]
[http://2010.igem.org/Team:Tsinghua/Notebook/30_August_2010 30] [http://2010.igem.org/Team:Tsinghua/Notebook/31_August_2010 31]
September
MTWTFSS
    [http://2010.igem.org/Team:Tsinghua/Notebook/1_September_2010 1] [http://2010.igem.org/Team:Tsinghua/Notebook/2_September_2010 2] [http://2010.igem.org/Team:Tsinghua/Notebook/3_September_2010 3] [http://2010.igem.org/Team:Tsinghua/Notebook/4_September_2010 4] [http://2010.igem.org/Team:Tsinghua/Notebook/5_September_2010 5]
[http://2010.igem.org/Team:Tsinghua/Notebook/6_September_2010 6] [http://2010.igem.org/Team:Tsinghua/Notebook/7_September_2010 7] [http://2010.igem.org/Team:Tsinghua/Notebook/8_September_2010 8] [http://2010.igem.org/Team:Tsinghua/Notebook/9_September_2010 9] [http://2010.igem.org/Team:Tsinghua/Notebook/10_September_2010 10] [http://2010.igem.org/Team:Tsinghua/Notebook/11_September_2010 11] [http://2010.igem.org/Team:Tsinghua/Notebook/12_September_2010 12]
[http://2010.igem.org/Team:Tsinghua/Notebook/13_September_2010 13] [http://2010.igem.org/Team:Tsinghua/Notebook/14_September_2010 14] [http://2010.igem.org/Team:Tsinghua/Notebook/15_September_2010 15] [http://2010.igem.org/Team:Tsinghua/Notebook/16_September_2010 16] [http://2010.igem.org/Team:Tsinghua/Notebook/17_September_2010 17] [http://2010.igem.org/Team:Tsinghua/Notebook/18_September_2010 18] [http://2010.igem.org/Team:Tsinghua/Notebook/19_September_2010 19]
[http://2010.igem.org/Team:Tsinghua/Notebook/20_September_2010 20] [http://2010.igem.org/Team:Tsinghua/Notebook/21_September_2010 21] [http://2010.igem.org/Team:Tsinghua/Notebook/22_September_2010 22] [http://2010.igem.org/Team:Tsinghua/Notebook/23_September_2010 23] [http://2010.igem.org/Team:Tsinghua/Notebook/24_September_2010 24] [http://2010.igem.org/Team:Tsinghua/Notebook/25_September_2010 25] [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/26_September_2010&action=edit 26]
[http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/27_September_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/28_September_2010&action=edit 28] [http://2010.igem.org/Team:Tsinghua/Notebook/29_September_2010 29] [http://2010.igem.org/Team:Tsinghua/Notebook/30_September_2010 30]
October
MTWTFSS
        [http://2010.igem.org/Team:Tsinghua/Notebook/1_October_2010 1] [http://2010.igem.org/Team:Tsinghua/Notebook/2_October_2010 2] [http://2010.igem.org/Team:Tsinghua/Notebook/3_October_2010 3]
[http://2010.igem.org/Team:Tsinghua/Notebook/4_October_2010 4] [http://2010.igem.org/Team:Tsinghua/Notebook/5_October_2010 5] [http://2010.igem.org/Team:Tsinghua/Notebook/6_October_2010 6] [http://2010.igem.org/Team:Tsinghua/Notebook/7_October_2010 7] [http://2010.igem.org/Team:Tsinghua/Notebook/8_October_2010 8] [http://2010.igem.org/Team:Tsinghua/Notebook/9_October_2010 9] [http://2010.igem.org/Team:Tsinghua/Notebook/10_October_2010 10]
[http://2010.igem.org/Team:Tsinghua/Notebook/11_October_2010 11] [http://2010.igem.org/Team:Tsinghua/Notebook/12_October_2010 12] [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/13_October_2010&action=edit 13] [http://2010.igem.org/Team:Tsinghua/Notebook/14_October_2010 14] [http://2010.igem.org/Team:Tsinghua/Notebook/15_October_2010 15] [http://2010.igem.org/Team:Tsinghua/Notebook/16_October_2010 16] [http://2010.igem.org/Team:Tsinghua/Notebook/17_October_2010 17]
[http://2010.igem.org/Team:Tsinghua/Notebook/18_October_2010 18] [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/19_October_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/20_October_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/21_October_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/22_October_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/23_October_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/24_October_2010&action=edit 24]
[http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/25_October_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/26_October_2010&action=edit 26] [http://2010.igem.org/Team:Tsinghua/Notebook/27_October_2010 27] [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/28_October_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/29_October_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/30_October_2010&action=edit 30] [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/31_October_2010&action=edit 31]

Our Experiments were carried out by the following nine groups which are somewhat independent from each other. Here come the Groups and Their Tasks.
Group 1A：Landing pad (LP) construction and Insertion Project with Att Recomination method and Trandition Landing pad








Protocols

Molecular Cloning
THU_Protocol_1-1_Isolation_of_plasmid_DNA
THU_Protocol_1-3_Restriction_Enzyme_Digestion
THU_Protocol_1-4_Ligation_of_DNA_Fragments
THU_Protocol_1-10_Electro_Transformation_of_Recombinant_DNA
THU_Protocol_1-15_PCR

Protein Isolation and Identification

Results

Group 2(b)
PCR: Amplication eGFP, mCherry, Kanamycin resistant gene and Chlr four genes separatly from PIB-eGFP, PBS34, PKD13 and PKD3 plasmids.
[[Image:Fan1]] [[Image:Fan1.JPG]] [[Image:Fan2.jpg|after the gel extraction]]

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