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Team:Caltech/BBa K338003
From 2010.igem.org
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===Usage and Biology=== | ===Usage and Biology=== | ||
====Design==== | ====Design==== | ||
| - | When ligated upstream of [[Team:Caltech/BBa_K338004|BBa_K338004]], the completed construct was designed to express all three PHA synthase genes required to make PHB from soybean oil. | + | When ligated upstream of [[Team:Caltech/BBa_K338004|BBa_K338004]], the completed construct was designed to express all three PHA synthase genes required to make PHB oligomers from soybean oil. The three genes would be transcribed polycistronically on a single mRNA transcript under the IPTG-inducible control of the <partinfo>BBa_K215000</partinfo> promoter. Naturally, each gene is preceded by a standard RBS (<partinfo>B0034</partinfo>) and the transcript finishes with a strong terminator (<partinfo>B0015</partinfo>), for a total size of about 3500bp. |
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| + | Note that these three genes should only cause the production of PHB ''oligomers'' in cells, not hardened plastic. A crosslinking agent is required to link the oligomers and form the final plastic product. Over-expression of the ''phaC1'' gene could cause some crosslinking, but this has not been experimentally verified. | ||
====BioBrick Characterization==== | ====BioBrick Characterization==== | ||
Revision as of 10:27, 26 October 2010
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 <partinfo>BBa_K338003 short</partinfo> This is half of a planned part which would contain all three PHA synthase genes required to produce [http://en.wikipedia.org/wiki/Polyhydroxybutyrate polyhydroxybutyrate] (PHB) in cells: phaA, phaB1, phaC1. This half contains only phaA: <partinfo>BBa_K156012</partinfo>. It was designed to be ligated upstream of part BBa_K338004. Usage and BiologyDesignWhen ligated upstream of BBa_K338004, the completed construct was designed to express all three PHA synthase genes required to make PHB oligomers from soybean oil. The three genes would be transcribed polycistronically on a single mRNA transcript under the IPTG-inducible control of the <partinfo>BBa_K215000</partinfo> promoter. Naturally, each gene is preceded by a standard RBS (<partinfo>B0034</partinfo>) and the transcript finishes with a strong terminator (<partinfo>B0015</partinfo>), for a total size of about 3500bp. Note that these three genes should only cause the production of PHB oligomers in cells, not hardened plastic. A crosslinking agent is required to link the oligomers and form the final plastic product. Over-expression of the phaC1 gene could cause some crosslinking, but this has not been experimentally verified. BioBrick CharacterizationEffect of Heat Shock TemperatureEffect of Heat Shock Duration
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