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Team:Chiba/Notebook 3
From 2010.igem.org
(Difference between revisions)
| Line 252: | Line 252: | ||
transformation | transformation | ||
</pre> | </pre> | ||
| + | |||
<html><h3>2010-10-13</h3></html> | <html><h3>2010-10-13</h3></html> | ||
| + | <pre> | ||
| + | Double transformation | ||
| + | ----------------------- | ||
| + | Plux inv-GFP 1 µL | ||
| + | Ptrc-LuxR 1 µL | ||
| + | ----------------------- | ||
| + | </pre> | ||
Revision as of 07:58, 26 October 2010
Lux inverter
2010-10-8
transformation BBa_R0061 BBa_E0240
2010-10-9
Mini-prep BBa_R0061 BBa_E0240 digestion -------------------- BBa_R0061 20 µL 10×Buffer 3 µL SpeI 1 µL PstI 1 µL NFW 5 µL -------------------- -------------------- BBa_E0240 20 µL 10×Buffer 3 µL XbaI 1 µL PstI 1 µL NFW 5 µL -------------------- ↓ gel extraction
2010-10-11
Ligation ----------------------- BBa_R0061 (70 ng) 1 µL BBa_E0240 (100 ng) 2 µL ATP (10 mM) 1 µL 10×Buffer 1 µL T4 DNA Ligase 1 µL NFW 4 µL ----------------------- ↓ transformation
2010-10-12
Ligation ----------------------- BBa_R0061(100 ng) 1 µL BBa_E0240 (100 ng) 3 µL ATP (10 mM) 1 µL T4 DNA Ligase 1 µL dH<sub>2</sub>O 4 µL ----------------------- ↓ transformation
2010-10-13
Double transformation ----------------------- Plux inv-GFP 1 µL Ptrc-LuxR 1 µL -----------------------
