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Team:Chiba/Notebook 3
From 2010.igem.org
(Difference between revisions)
| Line 188: | Line 188: | ||
transformation | transformation | ||
| - | + | ||
BBa_R0061 | BBa_R0061 | ||
BBa_E0240 | BBa_E0240 | ||
| - | + | ||
<br> | <br> | ||
| Line 200: | Line 200: | ||
digestion | digestion | ||
| - | |||
BBa_R0061 20 µL | BBa_R0061 20 µL | ||
10×Buffer 3 µL | 10×Buffer 3 µL | ||
| Line 224: | Line 223: | ||
Ligation | Ligation | ||
| - | + | BBa_R0061 (70 ng) 1 µL | |
| - | + | BBa_E0240 (100 ng) 2 µL | |
| - | ATP | + | ATP (10 mM) 1 µL |
| - | 10×Buffer | + | 10×Buffer 1 µL |
| - | T4 DNA Ligase | + | T4 DNA Ligase 1 µL |
| - | dH<sub>2</sub>O 4 µL | + | dH<sub>2</sub>O 4 µL |
transformation | transformation | ||
| Line 237: | Line 236: | ||
Ligation | Ligation | ||
| - | + | BBa_R0061(100 ng) 1 µL | |
| - | + | BBa_E0240 (100 ng) 3 µL | |
| - | ATP (10 mM) | + | ATP (10 mM) 1 µL |
| - | T4 DNA Ligase | + | T4 DNA Ligase 1 µL |
| - | dH<sub>2</sub>O 4 µL | + | dH<sub>2</sub>O 4 µL |
<html><h3>2010-10-13</h3></html> | <html><h3>2010-10-13</h3></html> | ||
Revision as of 06:16, 26 October 2010
Lux inverter
2010-10-8
transformation
BBa_R0061 BBa_E0240
2010-10-9
Mini-prep BBa_R0061 BBa_E0240digestion BBa_R0061 20 µL 10×Buffer 3 µL SpeI 1 µL PstI 1 µL dH2O5 µL
BBa_E0240 20 µL 10×Buffer 3 µL XbaI 1 µL PstI 1 µL dH2O 5 µL
gel extraction
2010-10-11
Ligation
BBa_R0061 (70 ng) 1 µL BBa_E0240 (100 ng) 2 µL ATP (10 mM) 1 µL 10×Buffer 1 µL T4 DNA Ligase 1 µL dH2O 4 µL
transformation
2010-10-12
Ligation
BBa_R0061(100 ng) 1 µL BBa_E0240 (100 ng) 3 µL ATP (10 mM) 1 µL T4 DNA Ligase 1 µL dH2O 4 µL
2010-10-13
